| Popis: |
Supplementary Figure 3: The cell growth inhibition and apoptosis by CBS9106 is suppressed by MLN4924 in MM.1S cells. (A) MM.1S cells are treated with the indicated concentration of CBS9106 and/or MLN4924 for 72 hours (n = 6). Cell viability is determined by cell counting kit-8. (B) Cells are treated with CBS9106 (50 nM) and/or MLN4924 (10 or 100 nM) for indicated period (48-96 hours, n = 6). Cell growth is determined by cell counting kit-8. (C) The apoptotic population (indicated by sub-G1) is measured by flow cytometry after staining with propidium iodide. MM.1S cells are treated with CBS9106 (50 or 100 nM), LMB (1 nM), or MLN4924 (1, 2, 5, 10, or 50 nM) either alone or in combination for 24 hours (n = 3). Data are expressed as the mean {plus minus} SD. *P |
