Differential binding of human nuclear proteins to Alu subfamilies
| Autor: | E. M. Bradbury, Nikolai V. Tomilin, C.W. Schmid, V. M. Bozhkov |
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| Rok vydání: | 1992 |
| Předmět: |
Electrophoresis
Genetics Subfamily Base Sequence Molecular Sequence Data Nuclear Proteins Alu element DNA Methylation Biology Molecular biology RNA polymerase III chemistry.chemical_compound Oligodeoxyribonucleotides CpG site chemistry Humans Electrophoretic mobility shift assay Nuclear protein Repetitive Sequences Nucleic Acid |
| Zdroj: | Nucleic Acids Research. 20:2941-2945 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/20.12.2941 |
| Popis: | Several diagnostic differences that distinguish human Alu subfamilies are clustered just downstream from the B box of the RNA polymerase III promoter; we tentatively refer to this diagnostic region as the DB box. Assuming that this region might determine the relative transcriptional activity of Alu subfamilies, we examined the interaction of nuclear proteins with DB box sequences representing different Alu subfamilies. Gel mobility shift assays suggest the existence of two factors which discriminate among the DB boxes of different Alu subfamilies: 1) An abundant, ca. 50 kd, protein binds more stably to a young 'PV' Alu subfamily (PVS) than to the older major subfamily (MS). 2) Methylation of CpG dinucleotides stimulates the binding of a less abundant, ca. 70 kd, protein to the DB boxes of younger Alu subfamilies. |
| Databáze: | OpenAIRE |
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