A sensitive sperm-motility test for the assessment of cytotoxic effect of pesticides
| Autor: | K Bertheussen, Mokhtar I. Yousef, S Helmi, M A Seehy, H. Z. Ibrahim, M. H. Salem |
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| Rok vydání: | 1996 |
| Předmět: |
Male
endocrine system Insecticides Cell Survival Glycine Motility Semen Biology Sensitivity and Specificity Toxicology Andrology chemistry.chemical_compound Carbofuran Bioassay Animals Humans reproductive and urinary physiology Sperm motility Lagomorpha urogenital system Herbicides Reproduction General Medicine biology.organism_classification Pollution Sperm Culture Media chemistry Evaluation Studies as Topic Toxicity Sperm Motility Rabbits Food Science |
| Zdroj: | Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes. 31(1) |
| ISSN: | 0360-1234 |
| Popis: | A sensitive sperm-motility test for the evaluation of cytotoxic effects of carbofuran and glyphosate in a defined protein-free culture medium is described. The sperm motility was compared to that obtained with a protein-containing medium. The use of protein-free medium considerably increased the sensitivity of sperm cells from rabbit and human to the toxic effects of the pesticide. The respective IC50 values (the concentration needed to cause 50% inhibition of sperm motility) in protein-free medium of carbofuran and glyphosate were 321 and 48.2 microM with human sperm, and 116 and 23.5 microM with rabbit sperm. Whereas, the corresponding values in protein-containing medium were 920 and 740 microM, and 910 and 500 microM with human and rabbit sperm, respectively. Our results show that testing human and rabbit sperm in protein-free medium proves to be a more sensitive method than that in protein-containing medium. Additionally, the use of rabbit sperm is a more sensitive test system than human sperm. This study suggests that the rabbit sperm test appears to have a potential for the assessment of toxicity on human reproduction. |
| Databáze: | OpenAIRE |
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