High-resolution mapping of QTL for fatty acid composition in soybean using specific-locus amplified fragment sequencing

Autor: Shurong Yan, Fukuan Yu, Fenxia Han, Shengrui Zhang, Sun Junming, Bin Li, Ying Chen, Lianzheng Wang, Fan Shengxu
Rok vydání: 2016
Předmět:
Zdroj: TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
ISSN: 1432-2242
Popis: Key message We constructed a high-density linkage map comprising 3541 markers developed by specific-locus amplified fragment sequencing, and identified 26 stable QTL including nine novel loci, for fatty acid composition in soybean. Abstract Soybean oil quality and stability are mainly determined by the fatty acid composition of the seed. In the present study, we constructed a high-density genetic linkage map using 200 recombinant inbred lines derived from a cross between cultivated soybean varieties Luheidou2 and Nanhuizao, and SNP markers developed by specific-locus amplified fragment sequencing (SLAF-seq). This map comprises 3541 markers on 20 linkage groups and spans a genetic distance of 2534.42 cM, with an average distance of 0.72 cM between adjacent markers. Inclusive composite interval mapping revealed 26 stable QTL for five fatty acids, explaining 0.4–37.0% of the phenotypic variance for individual fatty acids across environments. Of these QTL, nine are novel loci (qLA1, qLNA2_1, qPA4_1, qLA4_1, qPA6_1, qSA12_1, qPA16_1, qOA18_1, and qFA19_1). These stable QTL harbor three fatty acid biosynthesis genes (GmFabG, GmACP, and GmFAD8), and 66 genes encoding lipid-related transcription factors. These stable QTL and tightly linked SNP markers can be used for marker-assisted selection in soybean breeding programs. Electronic supplementary material The online version of this article (doi:10.1007/s00122-017-2902-8) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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