Full-length genome sequences of the first H9N2 avian influenza viruses isolated in the Northeast of Algeria

Autor: Amine Boudaoud, N. Alloui, Abdeljelil Ghram, Jihene Lachheb, Mariette F. Ducatez, Josianne Loupias, Abdelheq Barberis, Angelina Gorrill
Přispěvatelé: Centre de Recherche de Biotechnologie, Partenaires INRAE, University of Batna Hadj Lakhder [Algeria], Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP), Veterinary Department, ISVSA, University of Batna and the Laboratory of Epidemiology and Veterinary Microbiology, Pasteur Institute of Tunis, University of Tunis, El Manar, Tunisia, sponsored the screening of the different strains. IHAP, University of Toulouse, INRAE, ENVT, supported virus isolation and full genome sequencing.
Rok vydání: 2020
Předmět:
Zdroj: Virology Journal
Virology Journal, BioMed Central, 2020, 17 (1), pp.108. ⟨10.1186/s12985-020-01377-z⟩
Virology Journal, Vol 17, Iss 1, Pp 1-16 (2020)
ISSN: 1743-422X
DOI: 10.1186/s12985-020-01377-z
Popis: Background H9N2 avian influenza viruses (AIV) has a worldwide geographic distribution and affects poultry of different types of production. H9N2 AIV was first reported in the Northeast of Algeria in April 2017, following an outbreak associated with high mortality, in broiler flocks. In the present study, we report full-length genome sequences of AIV H9N2, and the detailed phylogeny and molecular genetic analyses. Methods Ten AIV H9N2 strains, collected in broiler flocks, were amplified in 9-day-old embryonated specific pathogen free (SPF) chicken eggs. Their full-length genomes were successfully sequenced and phylogenetic and molecular characterizations were conducted. Results Phylogenetic analysis showed that the isolates were monophyletic, grouped within the G-1 lineage and were very close to Moroccan and Algerian strains identified in 2016 and 2017, respectively. The low pathogenicity of the strains was confirmed by the sequence motif (335RSSR/GLF341) at the hemagglutinin (HA) cleavage site. An exclusive substitution (T197A) that had not been previously reported for H9N2 viruses; but, conserved in some pandemic H1N1 viruses, was observed. When compared to the G1-like H9N2 prototype, the studied strains showed one less glycosylation site in HA, but 2–3 additional ones in the stalk of the neuraminidase (NA). The HA protein harbored the substitution 234 L, suggesting binding preference to human-like receptors. The NA protein harbored S372A and R403W substitutions, previously detected in H9N2 from Asia and the Middle East, and especially in H2N2 and H3N2 strains that caused human pandemics. Different molecular markers associated with virulence and mammalian infections have been detected in the viral internal proteins. The matrix M2 protein possessed the S31N substitution associated with drug resistance. The non-structural 1 (NS1) protein showed the “GSEV” PDZ ligand (PL) C-terminal motif and no 80–84 deletion. Conclusion Characterized Algerian AIV isolates showed mutations that suggest increased zoonotic potential. Additional studies in animal models are required to investigate the pathogenicity of these H9N2 AIV strains. Monitoring their evolution in both migratory and domestic birds is crucial to prevent transmission to humans. Implementation of adequate biosecurity measures that limit the introduction and the propagation of AIV H9N2 in Algerian poultry farm is crucial.
Databáze: OpenAIRE
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