13C-sucrose breath test: novel use of a noninvasive biomarker of environmental gut health

Autor: Geoffrey P. Davidson, Cuong D. Tran, Yvette McNeil, Joanna S. Hawkes, Ross N. Butler, David Brewster, Brett Ritchie
Přispěvatelé: Ritchie, Brett K, Brewster, David R, Davidson, Geoffrey P, Tran, Cuong D, McNeil, Yvette, Hawkes, Joanna S, Butler, Ross N
Rok vydání: 2009
Předmět:
Male
Sucrose
Cell Membrane Permeability
Native Hawaiian or Other Pacific Islander
Gastroenterology
Rhamnose
Environmental Illness
Lactulose
Reference Values
Cardiovascular Medicine and Haematology
Intestine
Small
South Australia
Carbon Radioisotopes
Australian Aborigines
Intestinal Mucosa
medicine.diagnostic_test
Dehydration
Diarrhea
Breath Tests
Child
Preschool
Biomarker (medicine)
Female
medicine.symptom
medicine.drug
medicine.medical_specialty
tropical enteropathy
Asymptomatic
gut function
Sprue
Tropical
Diagnosis
Differential
Predictive Value of Tests
Internal medicine
medicine
Northern Territory
Blood test
Humans
tropic sprue
Breath test
Environmental enteropathy
Intestinal permeability
business.industry
sucrose breath test
intestinal permeability
Infant
medicine.disease
Intestinal Absorption
Pediatrics
Perinatology and Child Health
Immunology
Diarrhea
Infantile
environmental enteropath
business
Zdroj: Pediatrics. 124(2)
ISSN: 1098-4275
Popis: OBJECTIVE: Environmental enteropathy syndrome may compromise growth and predispose to infectious diseases in children in the developing world, including Australian Aboriginal children from remote communities of the Northern Territory. In this study, we described the use of a biomarker 13C-sucrose breath test (SBT) to measure enterocyte sucrase activity as a marker of small intestinal villus integrity and function. METHODS: This was a hospital-based prospective case-control study of Aboriginal and non-Aboriginal children with and without acute diarrheal disease. Using the SBT, we compared 36 Aboriginal case subjects admitted to a hospital (18 diarrheal and 18 nondiarrheal disease), with 7 healthy non-Aboriginal control subjects. Intestinal permeability using the lactulose/rhamnose (L/R) ratio on a timed 90-minute blood test was performed simultaneously with the SBT. The SBT results are expressed as a cumulative percentage of the dose recovered at 90 minutes. RESULTS: Aboriginal children with acute diarrheal disease have a significantly decreased absorptive capacity, as determined by the SBT, with a mean of 1.9% compared with either Aboriginal children without diarrhea (4.1%) or non-Aboriginal (6.1%) control subjects. The mean L/R ratio in the diarrhea group was 31.8 compared with 11.4 in Aboriginal children without diarrhea. There was a significant inverse correlation between the SBT and the L/R ratio. CONCLUSIONS: The SBT was able to discriminate among Aboriginal children with diarrhea, asymptomatic Aboriginal children with an underlying environmental enteropathy, and healthy non-Aboriginal controls. This test provides a noninvasive, easy-to-use, integrated marker of the absorptive capacity and integrity of the small intestine and could be a valuable tool in evaluating the efficacy of interventions aimed at improving gut health.
Databáze: OpenAIRE
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