3D FISH for the quantification of methane- and sulphur-oxidizing endosymbionts in bacteriocytes of the hydrothermal vent mussel Bathymodiolus azoricus
| Autor: | Sébastien Halary, Sébastien Duperron, Thomas Boudier, Françoise Gaill, Virginie Riou |
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| Přispěvatelé: | Systématique, adaptation, évolution (SAE), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Biologie intégrative (FRBI), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Imagerie intégrative de la molécule à l'organisme, Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Oceanography and Fisheries, University of the Azores, Analytical, Environmental and Geo- Chemistry, Vrije Universiteit Brussel (VUB), Neurobiologie des signaux intercellulaires (NSI), ANR Deep Oases/GDRE DIWOOD, Chemistry, Leballeur, Philippe, Institut de Biologie Intégrative (IFR-BI), Université Pierre et Marie Curie - Paris 6 (UPMC)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2008 |
| Předmět: |
MESH: Oxidation-Reduction
Gills 0106 biological sciences Bathymodiolus bacteriocyte MESH: Bacteriological Techniques sulphur-oxidizing bacteria 01 natural sciences MESH: Animals MESH: In Situ Hybridization Fluorescence In Situ Hybridization Fluorescence 0303 health sciences MESH: Symbiosis Ecology MESH: Sulfur MESH: Epithelial Cells methane-oxidizing bacteria Environmental chemistry Methane Oxidation-Reduction Hydrothermal vent animal structures MESH: Imaging Three-Dimensional Biology Microbiology 03 medical and health sciences Imaging Three-Dimensional Symbiosis [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Seawater 14. Life underwater Relative species abundance Ecology Evolution Behavior and Systematics 030304 developmental biology Bacteriological Techniques MESH: Mytilidae Bacteria 010604 marine biology & hydrobiology Bacteriocyte fungi MESH: Seawater Epithelial Cells Mussel biology.organism_classification 3D fluorescence in situ hybridization Cold seep MESH: Bacteria Mytilidae MESH: Methane MESH: Gills Sulfur |
| Zdroj: | Europe PubMed Central ISME Journal ISME Journal, Nature Publishing Group, 2008, 2(3), pp.284-292 international society for microbiological ecology international society for microbiological ecology, 2008, 2 (3), pp.284-92. ⟨10.1038/ismej.2008.3⟩ ISME Journal, 2008, 2(3), pp.284-292 |
| ISSN: | 1751-7370 1751-7362 |
| DOI: | 10.1038/ismej.2008.3 |
| Popis: | International audience; Dual endosymbioses involving methane- and sulphur-oxidizing bacteria occur in the gills of several species of mussels from deep-sea hydrothermal vents and cold seeps. Variations of total and relative abundances of symbionts depending on local environmental parameters are not yet understood, due to a lack of reliable quantification of bacteria in the host tissue. Here, we report the first attempt to quantify volumes occupied by each type of symbiont in bacteriocyte sections from a vent mussel, Bathymodiolus azoricus, using fluorescence in situ hybridization (FISH) coupled to three dimentional microscopy and image analysis carried out by a dedicated software, which we developed. Bacteriocytes from mussels recovered at different vent sites displayed significantly different abundances of bacteria. Specimens kept in aquaria at atmospheric pressure and exposed to an artificial pulse of sulphur displayed an increase in absolute and relative abundance of sulphur oxidizers within their bacteriocytes. Distributions of all measured parameters fitted normal distributions, indicating that bacteriocytes from a specimen tend to display similar behaviours. This study shows that symbiont volume quantification is tractable using 3D FISH, and confirms the impact of local environmental parameters on symbiont abundances. |
| Databáze: | OpenAIRE |
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