Effects of a Nutrient-Surfactant Compound on Solubilization Rates of TCE
| Autor: | Janet M. Strong-Gunderson, M. T. Gillespie |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Colony-forming unit
Aqueous solution Chromatography biology Chemistry Aqueous two-phase system Bioengineering General Medicine Biodegradation biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Nutrient Pulmonary surfactant Solubility Molecular Biology Bacteria Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. :835-843 |
| ISSN: | 1559-0291 0273-2289 |
| Popis: | BioTreat, a commercially available nutrient-surfactant compound, was investigated for its ability to solubilize TCE. Potential mechanisms for enhancing biodegradation rates by the use of nutrient-surfactant mixtures are: increased solubilization of TCE into the aqueous phase, and increased nutrients for the bacteria and greater numbers of colony forming units (CFUs). In aqueous systems, no measured solubilization of 0.1 and 1.0 ppm TCE from the headspace into the liquid phase was observed with BioTreat added at concentrations0.5%. However, at BioTreat concentrations in excess of the CMC (/=0.5%), increased solubilization of TCE was measured. A second question was the nutrient effect of BioTreat on the growth of the TCE-degrading bacterium, Burkholderia cepacia G4 PR1301. The added nutrients provided by BioTreat was evident and lead to increased cell numbers. The effect of BioTreat on the expression of ortho-monooxgenase, the enzyme necessary for TCE degradation by B. cepacia was also investigated. Enzyme expression as detected by a colorimetric assay was inhibited for BioTreat concentrations0.05%. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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