Pre-purification by membrane filtration of paralytic shellfish toxins from Alexandrium minutum dinoflagellate
| Autor: | Véronique Séchet, Zouher Amzil, Jiaxuan Zhang, Nicolas Brodu, Rafik Balti, Delphine Drouin, Anthony Massé |
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| Přispěvatelé: | Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Phytoxins laboratory, Nantes, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Bioprocédés Appliqués aux Microalgues (GEPEA-BAM), Laboratoire de génie des procédés - environnement - agroalimentaire (GEPEA), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Université de Nantes (UN)-Institut Universitaire de Technologie - Nantes (IUT Nantes), Université de Nantes (UN)-Institut Universitaire de Technologie Saint-Nazaire (IUT Saint-Nazaire), Université de Nantes (UN)-Institut Universitaire de Technologie - La Roche-sur-Yon (IUT La Roche-sur-Yon), Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Université Bretagne Loire (UBL)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Université Bretagne Loire (UBL), Laboratoire Phycotoxines |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Alexandrium minutum
Ultrafiltration Filtration and Separation 02 engineering and technology Analytical Chemistry Divalent law.invention [SPI]Engineering Sciences [physics] 020401 chemical engineering law Paralytic shellfish poisoning medicine [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering 0204 chemical engineering Filtration Purification ComputingMilieux_MISCELLANEOUS chemistry.chemical_classification Chromatography biology Dinoflagellate Membrane 021001 nanoscience & nanotechnology medicine.disease biology.organism_classification 6. Clean water Nanofiltration Shellfish poisoning Diafiltration chemistry 0210 nano-technology |
| Zdroj: | Separation and Purification Technology Separation and Purification Technology, Elsevier, 2019, 210, pp.152-158. ⟨10.1016/j.seppur.2018.07.063⟩ Separation And Purification Technology (1383-5866) (Elsevier Science Bv), 2019-02, Vol. 210, P. 152-158 |
| ISSN: | 1383-5866 |
| DOI: | 10.1016/j.seppur.2018.07.063⟩ |
| Popis: | The Paralytic shellfish neurotoxins (PST) are of increasing interest for biomedical applications. The chemical synthesis is often complex and expensive that’s why the purification by membrane filtration of PST from Alexandrium minutum dinoflagellate was investigated. Disrupted micro-alga cells by ultrasonic treatment were diafiltred to let pass toxins through an ultrafiltration membrane. Then, the mean permeate was concentrated and diafiltrated by nanofiltration. Mean permeate fluxes equal to 187, 135 and 135 L h−1 m−2 were obtained during the first diafiltration, the concentration step and the final diafiltration respectively. Up to 57% (mol/mol) and 78% (mol/mol) of organic matters and salts were removed respectively. Divalent ions were sparsely removed contrary to monovalent ones. C1 and C2 toxins were successfully purified since more than 75% (mol/mol) were recovered. However, only 27 to 50% (mol/mol) of GTX2, GTX3 and STX were recovered. |
| Databáze: | OpenAIRE |
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