Induction of apoptosis in yeast byL-amino acid oxidase from the Malayan pit viperCalloselasma rhodostoma
| Autor: | Heike Fussi, Sudharsana Rao Ande, Michael Murkovic, Peter Macheroux, Kai-Uwe Fröhlich, Heide Knauer, Sandro Ghisla |
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| Rok vydání: | 2008 |
| Předmět: |
Colony Count
Microbial Apoptosis Bioengineering Saccharomyces cerevisiae Viper Venoms Biology L-Amino Acid Oxidase L-amino-acid oxidase Applied Microbiology and Biotechnology Biochemistry chemistry.chemical_compound Leucine In Situ Nick-End Labeling Viperidae Genetics Animals Hydrogen peroxide chemistry.chemical_classification Oxidase test Malaysia Hydrogen Peroxide Catalase Molecular biology Yeast Enzyme chemistry Snake venom biology.protein Biotechnology |
| Zdroj: | Yeast. 25:349-357 |
| ISSN: | 1097-0061 0749-503X |
| Popis: | Here we report for the first time that L-amino acid oxidase (LAAO), a major component of snake venom, induces apoptosis in yeast. The causative agent for induction of apoptosis has been shown to be hydrogen peroxide, produced by the enzymatic activity of LAAO. However, the addition of catalase, a specific hydrogen peroxide scavenger, does not prevent cell demise completely. Intriguingly, depletion of leucine from the medium by LAAO and the interaction of LAAO with yeast cells are shown to be the major factors responsible for cell demise in the presence of catalase. |
| Databáze: | OpenAIRE |
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