Effect of heat treatment on cytotoxicity and polymerization of universal adhesives
| Autor: | Fernando Freitas Portella, Kathleen Sobieray, Daniel Rodrigo Marinowic, Celso Afonso Klein-Júnior, Roberto Zimmer, Eduardo Galia Reston, Keiichi Hosaka |
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| Rok vydání: | 2020 |
| Předmět: |
Hot Temperature
Materials science 0206 medical engineering Dental Bonding Dental Cements 030206 dentistry 02 engineering and technology 020601 biomedical engineering Polymerization Resin Cements 03 medical and health sciences 0302 clinical medicine Photopolymer Adhesives Dentin-Bonding Agents Dentin Materials Testing Air treatment Ceramics and Composites MTT assay Adhesive Cytotoxicity General Dentistry Nuclear chemistry |
| Zdroj: | Dental Materials Journal. 39:970-975 |
| ISSN: | 1881-1361 0287-4547 |
| Popis: | To assess, in vitro, the influence of heat air treatment on cytotoxicity and degree of conversion (DC) of universal self-etch adhesives (Ambar Universal APS, Scotchbond Universal Adhesive, and Tetric N-Bond Universal) in an NIH/3T3 fibroblast cell culture. Samples were divided into three groups: 1) no heat treatment (control), 2) 37°C and 3) 60°C heat treatment before photopolymerization. Cytotoxicity was analyzed by MTT assay and the DC by FTIR. All adhesives heated at 60°C showed reduced cytotoxicity levels when compared with those heated at 37°C. In general, DC of Ambar Universal APS presented the highest DC than Scotchbond Universal Adhesive and Tetric N-Bond Universal, and the hot air treatment do not influence the conversion. Heat treatment at 60°C was able to reduce the cytotoxicity of universal self-etch adhesives, even, the heat treatment does not enhances the DC. |
| Databáze: | OpenAIRE |
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