Impact of homocysteine on vasculogenic factors and bone formation in chicken embryos
| Autor: | Yara Maria Rauh Müller, Dib Ammar, Aline Pertile Remor, Maria Luísa da Silveira Hahmeyer, Evelise Maria Nazari, Alexandra Latini, Gilian Fernando Bourckhardt, Manuela Sozo Cecchini |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Hyperhomocysteinemia Homocysteine Health Toxicology and Mutagenesis Osteocalcin Neovascularization Physiologic Chick Embryo Toxicology Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Calcification Physiologic Osteogenesis medicine Animals Endochondral ossification biology Chemistry Ossification Embryogenesis Cell Biology medicine.disease 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Toxicity biology.protein Angiogenesis Inducing Agents medicine.symptom Blood vessel |
| Zdroj: | Cell biology and toxicology. 35(1) |
| ISSN: | 1573-6822 |
| Popis: | Developmental endochondral ossification requires constant blood supply, which is provided by the embryonic vascular network. High levels of homocysteine (Hcy) have vasculotoxic properties, but it remains unclear how Hcy disrupts blood vessel formation in endochondral ossification. Thus, we investigated the toxicity of Hcy on contents of vasculogenic factors (VEGF, VCAM-1, NOS3) and osteocalcin, using developing limbs as model. Chicken embryos were submitted to treatment with 20 μmol D-L Hcy at 12H&H and the analyses occur at 29H&H and 36H&H. We did not identify differences in the area of limb ossification in Hcy-treated (7.5 × 105 μm2 ± 3.9 × 104) and untreated embryos (7.6 × 105 μm2 ± 3.3 × 104) at 36H&H. In Hcy-treated embryos, we observed a significantly decrease of 46.8% at 29H&H and 26.0% at 36H&H in the number of VEGF-reactive cells. Also, treated embryos showed decrease of 98.7% in VCAM-1-reactive cells at 29H&H and 34.6% at 36H&H. The number of NOS3-reactive cells was reduced 54.0% at 29H&H and 91.5% at 36H&H, in the limbs of Hcy-treated embryos. Finally, in Hcy-treated embryos at 36H&H, we observed a reduction of 58.86% in the number of osteocalcin-reactive cells. Here, we demonstrated for the first time that the toxicity of Hcy is associated with a reduction in the contents of proteins involved in blood vessel formation and bone mineralization, which interferes with endochondral ossification of the limb during embryonic development. |
| Databáze: | OpenAIRE |
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