The Renal Cortical Na + /HCO 3 − Cotransporter VI: The Effect of Chemical Modification in Cotransporter Activity
| Autor: | F. T. Kear, A. A. Bernardo, Jose A.L. Arruda |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
inorganic chemicals
Glycosylation Kidney Cortex Physiology Bicarbonate Sodium Carbohydrates Biophysics chemistry.chemical_element digestive system Dithiothreitol chemistry.chemical_compound Animals Sulfhydryl Compounds Tyrosine Binding site urogenital system Chemistry Sodium-Bicarbonate Symporters Cell Biology Tetranitromethane Dicyclohexylcarbodiimide Biochemistry Rabbits Carrier Proteins Cotransporter |
| Zdroj: | Journal of Membrane Biology. 158:49-57 |
| ISSN: | 1432-1424 0022-2631 |
| DOI: | 10.1007/s002329900242 |
| Popis: | The Na+/HCO3- cotransporter is the main system that mediates bicarbonate removal out of the proximal tubule cell into the blood. We have previously partially purified this protein and showed that chemical modification of the alpha-amino groups by fluorescein isothiocyanate (FITC) inhibited the activity of the Na+/HCO3- cotransporter. The inhibition was prevented by the presence of Na and bicarbonate suggesting that this compound binds at or near the substrate transport sites of the cotransporter. We examined the effect of agents that modify the sulfhydryl group (dithiothreitol), carboxyl groups (n-n'dicyclohexyl carbodiimide) and tyrosine residues (p-nitrobenzene sulfonyl fluoride, n-acetyl imidazole and tetranitromethane) on the activity of the cotransporter to gain insight into the chemical residues which may be important for transport function. The sulfhydryl residues modifier, carboxyl group modifier, and tyrosine modifier significantly inhibited bicarbonate dependent 22Na uptake in basolateral membranes by 50-70% without altering the 22Na uptake in the presence of gluconate indicating that these agents directly affected the cotransporter without affecting diffusive sodium uptake. The effect of the tyrosine modifier n-acetylimidazole was not prevented by the presence of Na and bicarbonate suggesting that the tyrosine residues are not at the substrate binding sites. To determine the presence and role of glycosylation on the Na+/HCO3- cotransporter protein, we examined the effects of different glycosidases (endoglycosidase F and H, N-glycosidase F, O-glycanase) on the cotransporter activity. All glycosidases caused a significant 50-80% inhibition of cotransporter activity. These data demonstrate that N-glycosylation as well as O-glycosylation are important for the function of the Na+/HCO3- cotransporter protein. Taken together, these results suggest that chemical modifiers of tyrosine, carboxyl and sulfhydryl groups as well as glycosylation are important for expression of full functional activity of the cotransporter. |
| Databáze: | OpenAIRE |
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