PML‐like subnuclear bodies, containing XRCC1, juxtaposed to DNA replication‐based single‐strand breaks
| Autor: | Jurek Dobrucki, Oskar Szelest, Kamil Solarczyk, Magdalena Tworzydło, Christoph Cremer, Magdalena Kordon, Krzysztof Berniak, Sebastian Wachsmann-Hogiu, Aleksander Szczurek, Anne Vaahtokari, Thoru Pederson |
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| Rok vydání: | 2018 |
| Předmět: |
DNA Replication
0301 basic medicine DNA Repair DNA damage Origin Recognition Complex Poly (ADP-Ribose) Polymerase-1 Promyelocytic Leukemia Protein Autoantigens Biochemistry 03 medical and health sciences XRCC1 chemistry.chemical_compound 0302 clinical medicine PARP1 Protein Domains Genetics Humans DNA Breaks Single-Stranded Molecular Biology Cells Cultured Cell Nucleus chemistry.chemical_classification DNA ligase Chemistry Research DNA replication Antigens Nuclear Base excision repair Cell biology X-ray Repair Cross Complementing Protein 1 030104 developmental biology Origin recognition complex 030217 neurology & neurosurgery DNA HeLa Cells Biotechnology |
| Zdroj: | The FASEB Journal. 33:2301-2313 |
| ISSN: | 1530-6860 0892-6638 |
| DOI: | 10.1096/fj.201801379r |
| Popis: | DNA lesions induce recruitment and accumulation of various repair factors, resulting in formation of discrete nuclear foci. Using superresolution fluorescence microscopy as well as live cell and quantitative imaging, we demonstrate that X-ray repair cross-complementing protein 1 (XRCC1), a key factor in single-strand break and base excision repair, is recruited into nuclear bodies formed in response to replication-related single-strand breaks. Intriguingly, these bodies are assembled immediately in the vicinity of these breaks and never fully colocalize with replication foci. They are structurally organized, containing canonical promyelocytic leukemia (PML) nuclear body protein SP100 concentrated in a peripheral layer, and XRCC1 in the center. They also contain other factors, including PML, poly(ADP-ribose) polymerase 1 (PARP1), ligase IIIα, and origin recognition complex subunit 5. The breast cancer 1 and -2 C terminus domains of XRCC1 are essential for formation of these repair foci. These results reveal that XRCC1-contaning foci constitute newly recognized PML-like nuclear bodies that accrete and locally deliver essential factors for repair of single-strand DNA breaks in replication regions.—Kordon, M. M., Szczurek, A., Berniak, K., Szelest, O., Solarczyk, K., Tworzydło, M., Wachsmann-Hogiu, S., Vaahtokari, A., Cremer, C., Pederson, T., Dobrucki, J. W. PML-like subnuclear bodies, containing XRCC1, juxtaposed to DNA replication-based single-strand breaks. |
| Databáze: | OpenAIRE |
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