Critical Values of Porosity in Rice Cultures of Isaria fumosorosea by Adding Water Hyacinth: Effect on Conidial Yields and Quality

Autor: Arturo Figueroa-Montero, Gustavo Viniegra-González, Ernesto Favela-Torres, Alejandro Angel-Cuapio, Isabelle Perraud-Gaime, Octavio Loera
Rok vydání: 2015
Předmět:
Zdroj: Applied Biochemistry and Biotechnology. 177:446-457
ISSN: 1559-0291
0273-2289
Popis: Conidia of the entomopathogenic fungus Isaria fumosorosea are used to control insect pests in crops. Commercially available mycoinsecticides manufactured with this fungus are produced on a large scale via solid-state cultures (SSC). In order to favour gaseous exchange in SCC, texturizers can be added to increase porosity fraction (epsilon). This work presents results of water hyacinth (Eichhornia crassipes) as a novel texturizer. A mixture of parboiled rice (PR), with a epsilon = 0.23, was used as a substrate, which was then mixed with water hyacinth (WH amendment) as a texturizer at different proportions affecting epsilon. Strains CNRCB1 and ARSEF3302 of I. fumosorosea yielded 1.6 (1.49-1.71) x 10(9) and 7.3 (7.02-7.58) x 10(9) conidia per gram of initial dry rice after 8 days, at epsilon values of 0.34 and 0.36, respectively. Improvement of conidial yields corresponded to 1.33 and 1.55 times, respectively, compared to rice alone using WH amendment in the mixtures PR:WH (%) at 90-10 and 80-20. In addition, infectivity against Galleria mellonella larvae was maintained. This is the first report of the use of water hyacinth as a texturizer in SSC, affecting epsilon, which is proposed a key parameter in conidia production by I. fumosorosea, without affecting conidial infectivity.
Databáze: OpenAIRE
Externí odkaz: