Comparative hazard identification by a single dose lung exposure of zinc oxide and silver nanomaterials in mice
| Autor: | Gosens, Ilse, Kermanizadeh, Ali, Jacobsen, Nicklas Raun, Lenz, Anke-Gabriele, Bokkers, Bas, de Jong, Wim H, Krystek, Petra, Tran, Lang, Stone, Vicki, Wallin, Håkan, Stoeger, Tobias, Cassee, Flemming R, LS IRAS EEPI Inhalatie Toxicologie, IRAS RATIA2, Risk Assessment of Toxic and Immunomodulatory Agents |
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| Přispěvatelé: | LS IRAS EEPI Inhalatie Toxicologie, IRAS RATIA2, Risk Assessment of Toxic and Immunomodulatory Agents |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Silver
Science Inflammation 02 engineering and technology 010501 environmental sciences Pharmacology Systemic inflammation 01 natural sciences Mice chemistry.chemical_compound Toxicity Tests medicine Animals Cytotoxic T cell Cytotoxicity Lung Cell damage Injections Spinal 0105 earth and related environmental sciences Multidisciplinary Glutathione 021001 nanoscience & nanotechnology medicine.disease Nanostructures 3. Good health Mice Inbred C57BL Instillation Drug medicine.anatomical_structure chemistry Immunology Toxicity Medicine Zinc Oxide medicine.symptom 0210 nano-technology Bronchoalveolar Lavage Fluid Research Article |
| Zdroj: | PLoS ONE 10:e0126934 (2015) PLoS ONE PLoS One, 10(5). Public Library of Science PLoS ONE, Vol 10, Iss 5, p e0126934 (2015) |
| ISSN: | 1932-6203 |
| Popis: | Comparative hazard identification of nanomaterials (NMs) can aid in the prioritisation for further toxicity testing. Here, we assessed the acute lung, systemic and liver responses in C57BL/6N mice for three NMs to provide a hazard ranking. A silver (Ag), non-functionalised zinc oxide (ZnO) and a triethoxycaprylylsilane functionalised ZnO NM suspended in water with 2% mouse serum were examined 24 hours following a single intratracheal instillation (I.T.). An acute pulmonary inflammation was noted (marked by a polymorphonuclear neutrophil influx) with cell damage (LDH and total protein) in broncho-alveolar lavage fluid (BALF) after administration of both non-functionalised and functionalised ZnO. The latter also induced systemic inflammation measured as an increase in blood neutrophils and a decrease in blood lymphocytes. Exposure to Ag NM was not accompanied by pulmonary inflammation or cytotoxicity, or by systemic inflammation. A decrease in glutathione levels was demonstrated in the liver following exposure to high doses of all three nanomaterials irrespective of any noticeable inflammatory or cytotoxic effects in the lung. By applying benchmark dose (BMD) modeling statistics to compare potencies of the NMs, we rank functionalised ZnO ranked the highest based on the largest number of affected endpoints, as well as the strongest responses observed after 24 hours. The non-functionalised ZnO NM gave an almost similar response, whereas Ag NM did not cause an acute response at similar doses. |
| Databáze: | OpenAIRE |
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