Delineating Astrocytic Cytokine Responses in a Human Stem Cell Model of Neural Trauma
| Autor: | Jamie S. Mitchell, Peter J. Hutchinson, Rickie Patani, Eric Peter Thelin, Susan Giorgi-Coll, Giulia E. Tyzack, Keri L. H. Carpenter, Adel Helmy, Arvid Frostell, Tamara Tajsic, Claire E. Hall, Aftab Alam |
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| Přispěvatelé: | Thelin, Eric [0000-0002-2338-4364], Alam, Mohammed [0000-0001-8089-6721], Carpenter, Keri [0000-0001-8236-7727], Hutchinson, Peter [0000-0002-2796-1835], Helmy, Adel [0000-0002-0531-0556], Apollo - University of Cambridge Repository |
| Rok vydání: | 2020 |
| Předmět: |
030506 rehabilitation
human iPSC-derived astrocytes Traumatic brain injury medicine.medical_treatment Induced Pluripotent Stem Cells TNF 03 medical and health sciences 0302 clinical medicine Immune system Neural Stem Cells TBI Brain Injuries Traumatic Medicine Humans Interleukin 6 Interleukin 4 Neuroinflammation Cells Cultured IL-6 biology business.industry IL-4 in vitro Original Articles medicine.disease 3. Good health cytokine exposure Interleukin 10 Cytokine IL-1β Astrocytes Immunology IL-10 biology.protein Cytokines Neurology (clinical) Stem cell 0305 other medical science business 030217 neurology & neurosurgery |
| Zdroj: | Journal of Neurotrauma |
| Popis: | Neuroinflammation has been shown to mediate the pathophysiological response following traumatic brain injury (TBI). Accumulating evidence implicates astrocytes as key immune cells within the central nervous system (CNS), displaying both pro- and anti-inflammatory properties. The aim of this study was to investigate how in vitro human astrocyte cultures respond to cytokines across a concentration range that approximates the aftermath of human TBI. To this end, enriched cultures of human induced pluripotent stem cell (iPSC)-derived astrocytes were exposed to interleukin-1β (IL-1β) (1–10,000 pg/mL), IL-4 (1–10,000 pg/mL), IL-6 (100–1,000,000 pg/mL), IL-10 (1–10,000 pg/mL) and tumor necrosis factor (TNF)-α (1–10,000 pg/mL). After 1, 24, 48 and 72 h, cultures were fixed and immunolabeled, and the secretome/supernatant was analyzed at 24, 48, and 72 h using a human cytokine/chemokine 39-plex Luminex assay. Data were compared to previous in vitro studies of neuronal cultures and clinical TBI studies. The secretome revealed concentration-, time- and/or both concentration- and time-dependent production of downstream cytokines (29, 21, and 17 cytokines, respectively, p |
| Databáze: | OpenAIRE |
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