Ontogenesis of myosin light chain kinase mRNA and protein content in guinea pig tracheal smooth muscle
| Autor: | Viviana Cantillana, Lu Wang, Pasquale Chitano, Thomas M. Murphy, Judith A. Voynow, Lauranell H. Burch, Valeria Pozzato |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Pulmonary and Respiratory Medicine
Male medicine.medical_specialty Pathology Myosin light-chain kinase Blotting Western Guinea Pigs macromolecular substances Biology Article Contractility Guinea pig Internal medicine medicine Animals RNA Messenger Myosin-Light-Chain Kinase chemistry.chemical_classification Messenger RNA Reverse Transcriptase Polymerase Chain Reaction Age Factors RNA Muscle Smooth Smooth muscle contraction Trachea Enzyme Endocrinology chemistry Pediatrics Perinatology and Child Health Female medicine.symptom Muscle contraction Muscle Contraction |
| Zdroj: | Pediatric pulmonology. 38(6) |
| ISSN: | 8755-6863 |
| Popis: | We previously reported in guinea pig tracheal smooth muscle that maximal shortening velocity decreases from 3 weeks of age to adulthood. It is not known whether myosin light chain kinase (MLCK), a key enzyme determining the velocity of smooth muscle contraction, undergoes maturational changes. In the present work, we investigated MLCK protein content and mRNA expression in 1-week-old, 3-week-old, and adult guinea pigs. We extracted either proteins or RNA from isolated tracheal smooth muscle. The content of MLCK was assessed by Western immunoblots. MLCK mRNA was evaluated by Northern analysis and by quantitative real time reverse transcriptase-polymerase chain reaction (RT-PCR). The content of MLCK increased 3-fold at 3 weeks of age and then decreased in adults, being 0.116 ± 0.042, 0.330 ± 0.125 (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text