Enhanced Pseudomonas aeruginosa Biofilm Development Mediated by Human Neutrophils
| Autor: | Michael B. Fessler, Jonathan G. Lieber, Jerry A. Nick, Kenneth C. Malcolm, Katie R. Poch, Milene T. Saavedra, Kerry L. Tomlin, Travis S. Walker, G. Scott Worthen, Michael L. Vasil |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Cystic Fibrosis
Neutrophils Immunology medicine.disease_cause Microbiology Cystic fibrosis Immune system Immunity medicine Humans Host Response and Inflammation biology Pseudomonas aeruginosa Biofilm DNA biology.organism_classification medicine.disease Actins Infectious Diseases Biofilms Sputum Parasitology medicine.symptom Bacteria Pseudomonadaceae |
| Popis: | Cystic fibrosis (CF) lung disease features persistent neutrophil accumulation to the airways from the time of infancy. CF children are frequently exposed toPseudomonas aeruginosa, and by adulthood, 80% of CF patients are chronically infected. The formation of biofilms is a particularly important phenotypic characteristic ofP. aeruginosathat allows for bacterial survival despite aggressive antibiotic therapy and an exuberant immune response. Here, we show that the presence of neutrophils enhances initialP. aeruginosabiofilm development over a period of 72 h through the formation of polymers comprised of actin and DNA. F-actin was found to be a site of attachment forP. aeruginosa. These actin and DNA polymers are present in CF sputum, and disruption of the polymers dispersed the associatedP. aeruginosacells and reduced biofilm development. These findings demonstrate a potential maladaptation of the primary innate response. When the host fails to eradicate the infection, cellular components from necrotic neutrophils can serve as a biological matrix to facilitateP. aeruginosabiofilm formation. |
| Databáze: | OpenAIRE |
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