Modulation of extracellular matrix metabolism in rabbit articular chondrocytes and human rheumatoid synovial cells by the non-steroidal anti-inflammatory drug etodolac. II: Glycosaminoglycan synthesis
| Autor: | Alain Mauviel, F. Redini, Pujol Jp, Loyau G |
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| Rok vydání: | 1990 |
| Předmět: |
Cartilage
Articular medicine.medical_treatment Immunology Connective tissue Osteoarthritis Pharmacology Toxicology Extracellular matrix Glycosaminoglycan Rheumatic Diseases medicine Animals Humans Pharmacology (medical) Etodolac Cells Cultured Glycosaminoglycans Indoleacetic Acids Chemistry Anti-Inflammatory Agents Non-Steroidal Synovial Membrane medicine.disease Extracellular Matrix medicine.anatomical_structure Cytokine Synovial Cell Rabbits Synovial membrane medicine.drug Interleukin-1 |
| Zdroj: | Agents and actions. 31(3-4) |
| ISSN: | 0065-4299 |
| Popis: | Cultures of human rheumatoid synovial cells and rabbit articular chondrocytes were exposed to various concentrations of Etodolac (from 0.01 to 10 μg/ml) in presence or absence of 500 pg/ml (5 U/ml) human recombinant Interleukin-1β (IL-1β). Incubation of chondrocytes with Etodolac for 24 h did not alter collagen biosynthesis. In contrast, 1 μg/ml Etodolac caused a 20% increase of collagen production in synoviocytes. Addition of Etodolac in combination with IL-1 could partially suppress the inhibitory effect exerted by the cytokine on both cell types. Four-day exposure of chondrocytes to 0.1 and 1 μg/ml Etodolac led to an increased accumulation of collagen in the cell layer compartment. However, this treatment could not prevent the inhibitory effect of IL-1 on this collagen fraction. Treatment of synoviocytes for eight days with the same concentrations of Etodolac did not modify their collagen production but suppressed totally the inhibitory effect of IL-1. These data show that Etodolac is able to augment chondrocyte metabolism during a long term treatment. Moreover, under certain conditions, this drug can reduce or even suppress the IL-1-induced inhibition of collagen biosynthesis, a process that may take a part in the connective tissue alterations associated with osteoarticular diseases such as rheumatoid arthritis and osteoarthritis. |
| Databáze: | OpenAIRE |
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