Comparative venomics of the Vipera ammodytes transcaucasiana and Vipera ammodytes montandoni from Turkey provides insights into kinship
| Autor: | Hempel, Benjamin-Florian, Damm, Maik, Göçmen, Bayram, Karis, Mert, Oguz, Mehmet Anıl, Nalbantsoy, Ayse, Süssmuth, Roderich D. |
|---|---|
| Přispěvatelé: | Ege Üniversitesi |
| Rok vydání: | 2018 |
| Předmět: |
Proteome
Turkey Cell Survival Vipera ammodytes Transcaucasian Nose-horned Viper Transdanubian Sand Viper Reptilian Proteins Viper Venoms The detailed characterization and comparison of the venom proteome Vipera ammodytes transcaucasiana and Vipera ammodytes montandoni showed an impressive matching of the venom composition which could help to overcome the question of the taxonomic status of Vipera ammodytestranscaucasiana. Additional bioactivity screenings against several cancerous and non-cancerous cell lines showed promising results against breast cancer cells Article intact mass profiling Cell Line Tumor tripeptide metalloprotease inhibitor 597 Wechselwarme Wirbeltiere Pisces (Fische) Viperidae snake venomics Animals Humans ddc:597 cytotoxicity ddc:610 610 Medizin und Gesundheit |
| Zdroj: | Toxins |
| DOI: | 10.14279/depositonce-6610 |
| Popis: | WOS: 000424096500023 PubMed ID: 29301241 The Nose-horned Viper (Vipera ammodytes) is one of the most widespread and venomous snakes in Europe, which causes high frequent snakebite accidents. The first comprehensive venom characterization of the regional endemic Transcaucasian Nose-horned Viper (Vipera ammodytes transcaucasiana) and the Transdanubian Sand Viper (Vipera ammodytes montandoni) is reported employing a combination of intact mass profiling and bottom-up proteomics. The bottom-up analysis of both subspecies identified the major snake protein families of viper venoms. Furthermore, intact mass profiling revealed the presence of two tripeptidic metalloprotease inhibitors and their precursors. While previous reports applied multivariate analysis techniques to clarify the taxonomic status of the subspecies, an accurate classification of Vipera ammodytestranscaucasiana is still part of the ongoing research. The comparative analysis of the viper venoms on the proteome level reveals a close relationship between the Vipera ammodytes subspecies, which could be considered to clarify the classification of the Transcaucasian Nose-horned Viper. However, the slightly different ratio of some venom components could be indicating interspecific variations of the two studied subspecies or intraspecies alternations based on small sample size. Additionally, we performed a bioactivity screening with the crude venoms against several human cancerous and non-cancerous cell lines, which showed interesting results against a human breast adenocarcinoma epithelial cell line. Several fractions of Vipera a. transcaucasiana demonstrated a strong cytotoxic effect on triple negative MDA MB 231 breast cancer cells. German Research FoundationGerman Research Foundation (DFG); Technische Universitat Berlin We thank Daniel Petras (University of California) and Rashed Al Toma (TU Berlin) for helpful discussion on the manuscript. We acknowledge support by the German Research Foundation and the Open Access Publication Funds of Technische Universitat Berlin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|