Current applications of orcein in histochemistry. A brief review with some new observations concerning influence of dye batch variation and aging of dye solutions on staining
| Autor: | Anthony Henwood |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Hepatitis B Surface Antigens
Time Factors Histology Chromatography Staining and Labeling Histocytochemistry Orcein stain Permanganate Mucins General Medicine Elastic Tissue Hepatitis b surface antigen Staining Medical Laboratory Technology chemistry.chemical_compound Biological stain Liver chemistry Metalloproteins Oxazines Orcein |
| Zdroj: | Biotechnic & Histochemistry. 78:303-308 |
| ISSN: | 1473-7760 1052-0295 |
| DOI: | 10.1080/10520290410001671335 |
| Popis: | Current uses of orcein to demonstrate elastic fibers and, following permanganate oxidation (Shikata's modification), hepatitis B surface antigen, copper associated protein, and sulfated mucins, are reviewed. Variations in staining performance with batch of dye and age of dye solution is also discussed. Additional experimental findings support the view that the orcein stain for elastic tissue and Shikata's modification produces consistent, high quality results as long as appropriate controls and suitable dye batches, e.g., Biological Stain Commission certified dyes, are used. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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