Biosynthesis of dopamine and serotonin in the rat brain after repeated cocaine injections: a microdissection mapping study
| Autor: | John S. Partilla, Thomas J. Raley, Michael H. Baumann, Richard B. Rothman |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
medicine.medical_specialty Serotonin Dopamine Caudate nucleus Decarboxylase inhibitor Nucleus accumbens Injections 5-Hydroxytryptophan Rats Sprague-Dawley Cellular and Molecular Neuroscience Cocaine Internal medicine medicine Electrochemistry Animals Chromatography High Pressure Liquid Chemistry Olfactory tubercle Dissection Brain Dihydroxyphenylalanine Rats Monoamine neurotransmitter medicine.anatomical_structure Endocrinology Basolateral amygdala medicine.drug |
| Zdroj: | Synapse (New York, N.Y.). 14(1) |
| ISSN: | 0887-4476 |
| Popis: | The purpose of the present study was to examine the effects of chronic cocaine on dopamine (DA) and serotonin (5-HT) synthesis in several rat brain regions implicated in drug reinforcement. Male rats were treated twice daily with cocaine (15 mg/kg, ip) or saline for 1 week. After 42 hr of abstinence, rats were challenged with either cocaine (15 mg/kg, ip) or saline, followed by the aromatic L-amino acid decarboxylase inhibitor 3-hydroxybenzylhydrazine (NSD-1015; 100 mg/kg, ip). Animals were decapitated 30 min after NSD-1015 and discrete brain regions were microdissected from 300 μm frozen sections. Postmortem tissue levels of 3, 4-dihydroxyphenylalanine (DOPA) and 5-hyroxytryptophan (5-HTP) were quantified by HPLC with electrochemical detection and used to estimate biosynthesis of DA and 5-HT, respectively. In chronic saline-treated rats, cocaine dramatically suppressed DA and 5-HT synthesis in all forebrain regions examined, including: medial prefrontal cortex, nucleus accumbens, caudate nucleus, olfactory tubercle, and basolateral amygdala. The degree of inhibition ranged from 35-65% and was more pronounced in 5-HT neurons compared to DA neurons in the same tissue sample. In general, chronic cocaine did not significantly alter basal levels of DOPA or 5-HTP; a notable exception was lateral hypothalamus, where chronic cocaine reduced basal DA synthesis to 75% of control. After repeated cocaine injections, the synthesisiinhibiting effect of a challenge injection of cocaine was attenuated in many brain areas. These data suggest that whereas acute cocaine decreases DA and 5-HT synthesis in forebrain, chronic cocaine is not neurotoxic to DA and 5-HT neurons. In addition, the mechanism(s) mediating cocaine-induced suppression of monoamine synthesis may become desensitized by chronic exposure to the drug. Published 1993 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text