Subcellular localization of the C 19 -steroid reductase activities of female rat liver
| Autor: | Naomichi Arimasa, Charles D. Kochakian |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Cytoplasm
Chromatography Paper medicine.medical_treatment Metabolite Clinical Biochemistry Mitochondria Liver Reductase Epiandrosterone Biology Biochemistry Steroid chemistry.chemical_compound Endocrinology medicine Animals Testosterone Androstenedione Molecular Biology Pharmacology Cell Nucleus Carbon Isotopes Androsterone Organic Chemistry Hydroxysteroid Dehydrogenases Hydrogen-Ion Concentration Subcellular localization Ketosteroids NAD Rats Paper chromatography Kinetics chemistry Liver Microsomes Liver Female Steroids Chromatography Thin Layer Oxidoreductases Androstanes NADP |
| Zdroj: | Steroids. 19(3) |
| ISSN: | 0039-128X |
| Popis: | The role of DPNH and TPNH in the reduction of [4- 14 C]-testosterone or [4- 14 C] and rostenedione by female rat liver homogenate and its subcellular fractions was studied. The extracted metabolites were separated by thin layer and paper chromatography. 5α-Androstane-3α, 17β-diol was the major metabolite. Androsterone and 5α-dihydrotestosterone occurred in lesser quantities and 5α-androstane-3β, 17β-diol, 5α-androstanedione and epiandrosterone in small amounts. Androstenedione (or testosterone) was produced only in the presence of DPNH. The omission of the coenzymes or TPNH-generating system resulted in only a trace amount of the metabolites. |
| Databáze: | OpenAIRE |
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