Membrane potential regulates Hedgehog signalling in the Drosophila wing imaginal disc
| Autor: | Iswar K. Hariharan, Maya Emmons-Bell |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Patched
Biochemistry Article Membrane Potentials 03 medical and health sciences 0302 clinical medicine Genetics Animals Drosophila Proteins Wings Animal Compartment (development) Hedgehog Proteins Membrane & Intracellular Transport Molecular Biology Hedgehog 030304 developmental biology Membrane potential 0303 health sciences Chemistry Depolarization Articles Hedgehog signaling pathway Cell biology Imaginal disc Imaginal Discs Drosophila membrane potential signaling Smoothened Development & Differentiation 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | EMBO Reports |
| ISSN: | 1469-3178 1469-221X |
| DOI: | 10.15252/embr.202051861 |
| Popis: | While the membrane potential of cells has been shown to be patterned in some tissues, specific roles for membrane potential in regulating signalling pathways that function during development are still being established. In the Drosophila wing imaginal disc, Hedgehog (Hh) from posterior cells activates a signalling pathway in anterior cells near the boundary which is necessary for boundary maintenance. Here, we show that membrane potential is patterned in the wing disc. Anterior cells near the boundary, where Hh signalling is most active, are more depolarized than posterior cells across the boundary. Elevated expression of the ENaC channel Ripped Pocket (Rpk), observed in these anterior cells, requires Hh. Antagonizing Rpk reduces depolarization and Hh signal transduction. Using genetic and optogenetic manipulations, in both the wing disc and the salivary gland, we show that membrane depolarization promotes membrane localization of Smoothened and augments Hh signalling, independently of Patched. Thus, membrane depolarization and Hh‐dependent signalling mutually reinforce each other in cells immediately anterior to the compartment boundary. Membrane depolarization can promote signaling via the Hedgehog pathway in the Drosophila wing disc. |
| Databáze: | OpenAIRE |
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