Monophosphoryl lipid A enhances nontypeable Haemophilus influenzae-specific mucosal and systemic immune responses by intranasal immunization
| Autor: | Takashi Hirano, Toshiaki Kawano, Munehito Moriyama, Masashi Suzuki, Satoru Kodama, Yoshinori Kadowaki, Taro Iwasaki |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Haemophilus Infections medicine.medical_treatment 030106 microbiology Monophosphoryl Lipid A Enzyme-Linked Immunosorbent Assay medicine.disease_cause Microbiology Haemophilus influenzae Mice 03 medical and health sciences Immune system Adjuvants Immunologic otorhinolaryngologic diseases medicine Animals Immunity Mucosal Administration Intranasal Mice Inbred BALB C Innate immune system biology business.industry General Medicine Antibodies Bacterial Otitis Media Lipid A 030104 developmental biology Otorhinolaryngology Immunization Pediatrics Perinatology and Child Health Immunology TLR4 biology.protein Female Antibody business Adjuvant |
| Zdroj: | International Journal of Pediatric Otorhinolaryngology. 97:5-12 |
| ISSN: | 0165-5876 |
| Popis: | Objective Acute otitis media (AOM) is one of the most common infectious diseases in children. Nontypeable Haemophilus influenzae (NTHi) is Gram-negative bacteria that are considered major pathogens of AOM and respiratory tract infections. In this study, we used monophosphoryl lipid A (MPL), a toll-like receptor (TLR) 4 agonist, as an adjuvant to induce mucosal immune responses against NTHi to enhance bacterial clearance from the nasopharynx. Methods Mice were administered 10 μg outer membrane protein (OMP) from NTHi and 0, 10, or 20 μg MPL intranasally once a week for 3 weeks. Control mice were administered phosphate-buffered saline alone. After immunization, these mice were challenged with NTHi. At 6 and 12 h after bacterial challenge, the mice were killed and nasal washes and sera were collected. The numbers of NTHi- and OMP-specific antibodies were quantified by enzyme-linked immunosorbent assay. Results The MPL 10 and 20 μg group produced a significant reduction in the number of bacteria recovered from the nasopharynx at 12 h after bacterial challenge compared to the control group. OMP-specific IgA titers were also augmented in the MPL groups compared to the control and OMP groups. Conclusion MPL is suitable for eliciting effective mucosal immune responses against NTHi in the nasopharynx. These results demonstrate the possibility of an adjuvant that involves stimulation of the innate immune system by TLR4 agonists such as MPL for mucosal vaccination. |
| Databáze: | OpenAIRE |
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