Cry1Ac and Vip3Aa proteins from Bacillus thuringiensis targeting Cry toxin resistance in Diatraea flavipennella and Elasmopalpus lignosellus from sugarcane
| Autor: | Rebeka da Costa Alves, Ana Rita Nunes Lemes, Janete Apparecida Desidério, Liliane Marques da Silva, Isis Sebastião, Camila Soares Figueiredo, Odair Aparecido Fernandes, Manoel Victor Franco Lemos, Herbert A.A. Siqueira |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Federal Rural de Pernambuco |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
lcsh:Medicine Heterologous medicine.disease_cause General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Insecticidal proteins Bacillus thuringiensis medicine Genetics Mode of action Agricultural Science Escherichia coli biology Toxin General Neuroscience lcsh:R fungi Competition assay General Medicine biology.organism_classification Diatraea flavipennella 030104 developmental biology Biochemistry Cry1Ac Biotinylation Biological control General Agricultural and Biological Sciences Entomology Biotechnology |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP PeerJ PeerJ, Vol 5, p e2866 (2017) |
| Popis: | Made available in DSpace on 2018-12-11T17:09:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-01-01 The biological potential of Vip and Cry proteins from Bacillus is well known and widely established. Thus, it is important to look for new genes showing different modes of action, selecting those with differentiated entomotoxic activity against Diatraea flavipennella and Elasmopalpus lignosellus, which are secondary pests of sugarcane. Therefore, Cry1 and Vip3 proteins were expressed in Escherichia coli, and their toxicities were evaluated based on bioassays using neonate larvae. Of those, the most toxic were Cry1Ac and Vip3Aa considering the LC50 values. Toxins from E. coli were purified, solubilized, trypsinized, and biotinylated. Brush Border Membrane Vesicles (BBMVs) were prepared from intestines of the two species to perform homologous and heterologous competition assays. The binding assays demonstrated interactions between Cry1Aa, Cry1Ac, and Vip3Aa toxins and proteins from the BBMV of D. flavipennella and E. lignosellus. Homologous competition assays demonstrated that binding to one of the BBMV proteins was specific for each toxin. Heterologous competition assays indicated that Vip3Aa was unable to compete for Cry1Ac toxin binding. Our results suggest that Cry1Ac and Vip3Aa may have potential in future production of transgenic sugarcane for control of D. flavipennella and E. lignosellus, but more research is needed on the potential antagonism or synergism of the toxins in these pests. Department of Biologia Aplicada � Agropecu�ria Universidade Estadual Paulista Department of Agronomia Universidade Federal Rural de Pernambuco Departamento de Fitossanidade/FCAV/UNESP Universidade Estadual Paulista Department of Biologia Aplicada � Agropecu�ria Universidade Estadual Paulista Departamento de Fitossanidade/FCAV/UNESP Universidade Estadual Paulista |
| Databáze: | OpenAIRE |
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