Estrogen alters the effects of neuropeptide-Y on luteinizing hormone and follicle-stimulating hormone release in female rats at the level of the anterior pituitary gland
| Autor: | Gary T. Campbell, R. W. Steigerwalt, Charles A. Blake, Longping Liu, Michael J. Woller |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
endocrine system
Pituitary gland medicine.medical_specialty medicine.drug_class Gonadotropin-releasing hormone Gonadotropin-Releasing Hormone Follicle-stimulating hormone Endocrinology Anterior pituitary Pituitary Gland Anterior Internal medicine medicine Animals Neuropeptide Y Cells Cultured Chromatography High Pressure Liquid Chemistry Estrogens Rats Inbred Strains Luteinizing Hormone Rats medicine.anatomical_structure Estrogen Ovariectomized rat Female Follicle Stimulating Hormone Gonadotropin Luteinizing hormone hormones hormone substitutes and hormone antagonists |
| Zdroj: | Endocrinology. 133:2675-2681 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo.133.6.8243291 |
| Popis: | In recent years, several studies have shown that neuropeptide-Y (NPY) is involved in the control of LH secretion. We determined the effects of estrogen on NPY-induced LH and FSH release in the absence or presence of LH-releasing hormone (LHRH) at the level of the anterior pituitary gland (APG). Adult female rats were ovariectomized. Fifteen to 20 days later, they were given a blank or estrogen-filled capsule subdermally and killed 17-19 h later. APG cells were isolated and cultured for 3 days in medium containing 12.5% rat serum collected at death from the same rats used to make the respective APG cell pools. The cells were then challenged for 3 h with vehicle, NPY (10(-12)-10(-6) M), LHRH (10(-9)-10(-6) M), or combinations of NPY (10(-9)-10(-7) M) and LHRH (10(-9) M). LHRH stimulated LH and FSH release from nonestrogen and estrogen-primed cells. NPY at 6.7 x 10(-8)-10(-6) M increased (P < 0.05) LH release and at 10(-6) M increased (P < 0.05) FSH release from estrogen-primed cells, but was without effect on nonestrogen-primed cells. In contrast, NPY at 10(-9)-10(-7) M potentiated the action of LHRH (10(-9) M) to increase the release of LH and FSH from nonestrogen-primed cells, but was without potentiating effects in cultures of estrogen-primed cells. The results demonstrate that 1) NPY can release LH and FSH by a direct action on estrogen-primed APG cells; and 2) NPY can potentiate the action of LHRH to increase the release of LH and FSH by a direct action on nonestrogen-primed APG cells. |
| Databáze: | OpenAIRE |
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