Variability in platelet responses to collagen — comparison between whole blood perfusions, traditional platelet function tests and PFA-100
| Autor: | Martti Syrjälä, Pia Siljander, Aino Lepäntalo, Riitta Lassila, Jürg H. Beer |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Blood Platelets Male Integrins medicine.medical_specialty Receptors Collagen Epinephrine Platelet Aggregation Platelet Function Tests Citric Acid Amino Acid Chloromethyl Ketones Collagen receptor chemistry.chemical_compound Internal medicine medicine Von Willebrand disease Humans Platelet Receptor Aged Whole blood Polymorphism Genetic PFA-100 Anticoagulants Genetic Variation Reproducibility of Results Drug Synergism Hematology Middle Aged Platelet Activation medicine.disease Adenosine Diphosphate Perfusion Adenosine diphosphate Endocrinology chemistry Biochemistry Female Collagen Stress Mechanical Rheology |
| Zdroj: | University of Helsinki |
| ISSN: | 0049-3848 |
| DOI: | 10.1016/s0049-3848(01)00283-3 |
| Popis: | The purpose of this study was to determine if the results obtained in platelet function tests and whole blood perfusions are associated with those in platelet function analyser (PFA)-100. We used collagen type I monomers and fibrils to analyse the distinct roles of glycoprotein (GP) Ia/IIa and other collagen receptors in flowing blood under a high shear rate (1600/s) and in aggregation studies. Also, anticoagulation [citrate vs. d-phenylalanyl-1-prolyl-1 arginine chloromethyl ketone (PPACK)] was varied to enhance the functions of GP Ia/IIa, since it has been shown that the cation-poor environment of citrated blood impairs GP Ia/IIa-dependent platelet recruitment. Large interindividual variability (45-fold) was detected in deposition of platelets in whole blood perfusions over collagen monomers, whereas this variation was only fourfold in fibrils. In PFA, this variation was reduced to 2.5-fold. However, platelet deposition on monomers is associated with epinephrine-enhanced PFA (r=−.49, P |
| Databáze: | OpenAIRE |
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