Rapid detection and differentiation method of VanA, VanB and VanC phenotypes in vancomycin-resistant enterococci
| Autor: | Keisuke Sunakawa, Ariaki Nagayama, Hideaki Hanaki, Syuichi Nomura, Yoshio Yamaguchi |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Microbiology (medical)
DNA Bacterial medicine.drug_class Antibiotics Microbial Sensitivity Tests Biology Polymerase Chain Reaction law.invention Microbiology Bacterial Proteins law Vancomycin medicine Pharmacology (medical) Peptide Synthases Carbon-Oxygen Ligases Polymerase chain reaction Antibacterial agent Teicoplanin Streptococcus Drug Synergism Vancomycin Resistance General Medicine Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition bacterial infections and mycoses Streptococcaceae biology.organism_classification Glycopeptide Anti-Bacterial Agents carbohydrates (lipids) Infectious Diseases Phenotype Enterococcus Genes Bacterial bacteria medicine.drug |
| Zdroj: | International journal of antimicrobial agents. 23(5) |
| ISSN: | 0924-8579 |
| Popis: | We developed a simple method that can replace the polymerase chain reaction (PCR) to distinguish between vancomycin-resistant enterococci (VRE) with the vanA, vanB and vanC genes. The method is based on induction of teicoplanin resistance by vancomycin in vanB-VRE, while the two compounds have a synergistic effect in vanC-VRE. In addition, vanA-VRE shows resistance to both vancomycin and teicoplanin, and both the compounds can induce resistance to vanA-VRE. Utilising these properties, we attempted to develop a simple method to distinguish between vanA, vanB and vanC. We compared our simple method with the PCR method in 43 strains of vanA-VRE, 35 strains of vanB-VRE and 37 strains of vanC-VRE. The results were 100% consistent with that obtained by PCR. |
| Databáze: | OpenAIRE |
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