Serotype-independent detection of foot-and-mouth disease virus
| Autor: | Meng Yu, Jennifer A. McEachern, Jef M Hammond, Alfonso Clavijo, Janine D. Muller, Eric Hansson, Lin-Fa Wang, Katharine N. Bossart |
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| Rok vydání: | 2008 |
| Předmět: |
Serotype
viruses animal diseases Guinea Pigs Molecular Sequence Data Cattle Diseases Enzyme-Linked Immunosorbent Assay Antibodies Viral Antigen Antibody Specificity Virology Animals Amino Acid Sequence Serotyping Antigens Viral Viral Structural Proteins Antiserum Aphthovirus biology Immunogenicity biology.organism_classification Foot-and-Mouth Disease Virus Polyclonal antibodies Foot-and-Mouth Disease biology.protein Cattle Rabbits Foot-and-mouth disease virus Antibody |
| Zdroj: | Journal of Virological Methods. 151:146-153 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/j.jviromet.2008.03.011 |
| Popis: | Foot-and-mouth disease virus (FMDV) causes a highly contagious vesicular disease affecting cloven hoofed animals and is considered the most economically important disease worldwide. Recent FMD outbreaks in Europe and Taiwan and the associated need for rapid diagnostic turnaround have identified limitations that exist in current diagnostic capabilities. To aid improved diagnosis, a serotype-independent FMDV antigen capture assay was developed using antibodies directed against a highly conserved cross-reactive protein fragment (1AB') located within the structural protein 1AB. Cattle sera raised against all 7 serotypes of FMDV bound purified 1AB' demonstrating its immunogenicity in infected animals. Polyclonal anti-1AB' antiserum was produced in chickens and applied as a universal detector of FMDV antigen. Western blot analysis and ELISA both demonstrated that anti-1AB' serum could recognize FMDV antigens independent of serotype. Two recently characterized anti-FMDV monoclonal antibodies were also evaluated for their ability to capture FMDV antigen independently of serotype. When used in combination with chicken anti-1AB' antibodies in an antigen capture ELISA format, all serotypes of FMDV were detected. These data represent the first demonstration of the use of serotype-independent FMDV antigen capture reagents which may enable the development of rapid laboratory based assays or perhaps more significantly, rapid field-based pen-side or point of entry border control diagnostic tests. |
| Databáze: | OpenAIRE |
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