Hematocrit and standardization in DBS analysis: A practical approach for hormones mainly present in the plasma fraction
| Autor: | Mariëtte T. Ackermans, Vera de Kleijne, Annemieke C. Heijboer, Frans Martens |
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| Přispěvatelé: | Clinical chemistry, Amsterdam Gastroenterology Endocrinology Metabolism, Amsterdam Reproduction & Development (AR&D), Laboratory for Endocrinology, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Vitamin Clinical Biochemistry Dried blood spot Hematocrit Biochemistry behavioral disciplines and activities 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Tandem Mass Spectrometry medicine Humans Chromatography medicine.diagnostic_test business.industry Biochemistry (medical) General Medicine Venous blood Reference Standards Standardization Hormones 25OH-vitamin D nervous system diseases 030104 developmental biology surgical procedures operative chemistry nervous system 030220 oncology & carcinogenesis Plasma concentration Steroids Dried Blood Spot Testing business therapeutics Hormone |
| Zdroj: | Ackermans, M T, de Kleijne, V, Martens, F & Heijboer, A C 2021, ' Hematocrit and standardization in DBS analysis: A practical approach for hormones mainly present in the plasma fraction ', Clinica Chimica Acta, vol. 520, pp. 179-185 . https://doi.org/10.1016/j.cca.2021.06.014 Clinica Chimica Acta, 520, 179-185. Elsevier Clinica chimica acta; international journal of clinical chemistry, 520, 179-185. Elsevier |
| ISSN: | 0009-8981 |
| Popis: | Background and aim Dried blood spot (DBS) sampling has many advantages over conventionally used blood samples, but is thought to suffer from hematocrit related issues. The aim of our research was to investigate whether reliable results can be obtained without bothering about hematocrit effects in DBS analysis of analytes that are mainly present in the plasma compartment. Materials and Methods Venous blood samples with variation in hematocrit and spotted volume were prepared. Spot diameter and 25-OH Vitamin D3 and testosterone concentrations were measured. Moreover, DBS and plasma concentrations of 25-OH Vitamin D3, testosterone and hematocrit were determined in random patient samples. Results DBS spot size was linearly related to hematocrit. Measured DBS concentrations of 25-OH Vitamin D3 and testosterone were independent of hematocrit and spotted volume. Determining the relation between plasma and DBS concentration resulted in a factor that can be used to convert DBS concentrations to standardized plasma concentrations. Conclusion Addressing the hematocrit issue is not necessary for hormones that are mainly present in the plasma compartment. The relation between plasma and DBS concentration can be used to convert DBS concentrations to standardized plasma concentrations which makes interpretation of DBS concentrations easier. |
| Databáze: | OpenAIRE |
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