HPLC analysis as a tool for assessing targeted liposome composition
Autor: | Michael Platscher, Mira Oswald, Simon Geissler, Achim Goepferich |
---|---|
Rok vydání: | 2015 |
Předmět: |
Hplc analysis
Liposome Drug Carriers Chromatography Chemistry Pharmaceutical 010401 analytical chemistry Glycopeptides Pharmaceutical Science 02 engineering and technology Polyethylene glycol 021001 nanoscience & nanotechnology 01 natural sciences High-performance liquid chromatography 0104 chemical sciences chemistry.chemical_compound chemistry Chromatography detector Liposomes lipids (amino acids peptides and proteins) Composition (visual arts) 0210 nano-technology Drug carrier Control methods Chromatography High Pressure Liquid Phospholipids |
Zdroj: | International journal of pharmaceutics. 497(1-2) |
ISSN: | 1873-3476 |
Popis: | Functionalized phospholipids are indispensable materials for the design of targeted liposomes. Control over the quality and quantity of phospholipids is thereby key in the successful development and manufacture of such formulations. This was also the case for a complex liposomal preparation composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), Cholesterol (CHO), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG2000). To this end, an RP-HPLC method was developed. Detection was done via evaporative light scattering (ELS) for liposomal components. The method was validated for linearity, precision, accuracy, sensitivity and robustness. The liposomal compounds had a non-linear quadratic response in the concentration range of 0.012-0.42 mg/ml with a correlation coefficient greater than 0.99 with an accuracy of method confirmed 95-105% of the theoretical concentration. Furthermore, degradation products from the liposomal formulation could be identified. The presented method was successfully implemented as a control tool during the preparation of functionalized liposomes. It underlined the benefit of HPLC analysis of phospholipids during liposome preparation as an easy and rapid control method for the functionalized lipid at each preparation step as well as for the quantification of all components. |
Databáze: | OpenAIRE |
Externí odkaz: |