In vitro detection of circulating tumor cells compared by the CytoTrack and CellSearch methods
| Autor: | Thore Hillig, Sarah Nejlund, Peer Horn, Ann-Britt Nygaard, Anastasiya S. Haugaard, Ivan Brandslund, György Sölétormos |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Enumeration Breast Neoplasms Biology In Vitro Techniques CytoTrack Neoplasm genetics Andrology chemistry.chemical_compound Circulating tumor cell Antigen Recovery Antigens Neoplasm medicine Biomarkers Tumor Humans CellSearch Epithelial cell adhesion molecule General Medicine Epithelial Cell Adhesion Molecule Neoplastic Cells Circulating Prognosis CTC In vitro Flow sorting chemistry MCF-7 Cells Keratins Female Breast cancer cells Antigens neoplasm Cell Adhesion Molecules CTC Recovery Enumeration CytoTrack CellSearch METASTATIC BREAST-CANCER PREDICT PROGRESSION-FREE MESENCHYMAL TRANSITION SURVIVAL VALIDATION Research Article |
| Zdroj: | Tumour Biology Hillig, T, Horn, P, Nygaard, A-B, Haugaard, A S, Nejlund, S, Brandslund, I & Soletormos, G 2015, ' In vitro detection of circulating tumor cells compared by the CytoTrack and CellSearch methods ', Tumor Biology, vol. 36, no. 6, pp. 4597-4601 . https://doi.org/10.1007/s13277-015-3105-z |
| ISSN: | 1423-0380 1010-4283 |
| Popis: | Comparison of two methods to detect circulating tumor cells (CTC) CytoTrack and CellSearch through recovery of MCF-7 breast cancer cells, spiked into blood collected from healthy donors. Spiking of a fixed number of EpCAM and pan-cytokeratin positive MCF-7 cells into 7.5 mL donor blood was performed by FACSAria flow sorting. The samples were shipped to either CytoTrack or CellSearch research facilities within 48 h, where evaluation of MCF-7 recovery was performed. CytoTrack and CellSearch analyses were performed simultaneously. Recoveries of MCF-7 single cells, cells in clusters, and clusters were determined. The average numbers of MCF-7 cells/cells in clusters/clusters recovered from blood by the CytoTrack and CellSearch methods were 103 +/- 5.9/27 +/- 7.9/11 +/- 3.5 (95 % CI) and 107 +/- 4.4/20 +/- 7.1/10 +/- 3.5, respectively, with no difference between the two methods (p = 0.37/p = 0.23/p = 0.09). Overall, the recovery of CytoTrack and CellSearch was 68.8 +/- 3.9 %/71.1 +/- 2.9 %, respectively (p = 0.58). In spite of different methodologies, CytoTrack and CellSearch found similar number of CTCs, when spiking was performed with the EpCAM and pan cytokeratin-positive cell line MCF-7. The results suggest that CytoTrack and CellSearch have similar abilities to identify CTC in vitro. |
| Databáze: | OpenAIRE |
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