TheFTFgene family regulates virulence and expression of SIX effectors inFusarium oxysporum
| Autor: | Brisa Ramos, Serenella A. Sukno, Vega Tello, José J. de Vega-Bartol, Jonathan Niño-Sánchez, Jose Maria Diaz Minguez, Virginia Casado-Del Castillo |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Virulence Factors Genes Fungal Green Fluorescent Proteins Gene Dosage Soil Science Virulence Plant Science Biology Gene dosage Fungal Proteins 03 medical and health sciences Transformation Genetic Fusarium Solanum lycopersicum Gene Expression Regulation Fungal Sequence Homology Nucleic Acid Gene expression Pathogenicity Gene family Molecular Biology Gene Phylogeny Fusarium wilt Plant Diseases Genetics Gene knockdown 2409 Genética Effector food and beverages Fabaceae Original Articles FTF Blotting Southern 030104 developmental biology Multigene Family Host-Pathogen Interactions Mutation Original Article RNA Interference Transcription factor 2414 Microbiología Agronomy and Crop Science Genomic expansion |
| Zdroj: | GREDOS. Repositorio Institucional de la Universidad de Salamanca instname GREDOS: Repositorio Institucional de la Universidad de Salamanca Universidad de Salamanca (USAL) Molecular Plant Pathology |
| ISSN: | 1364-3703 1464-6722 |
| DOI: | 10.1111/mpp.12373 |
| Popis: | [EN] The FTF (Fusarium transcription factor) gene family comprises a single copy gene, FTF2, which is present in all the filamentous ascomycetes analysed, and several copies of a close relative, FTF1, which is exclusive to Fusarium oxysporum. An RNAmediated gene silencing system was developed to target mRNA produced by all the FTF genes, and tested in two formae speciales: F. oxysporum f. sp. phaseoli (whose host is common bean) and F. oxysporum f. sp. lycopersici (whose host is tomato). Quantification of the mRNA levels showed knockdown of FTF1 and FTF2 in randomly isolated transformants of both formae speciales. The attenuation of FTF expression resulted in a marked reduction in virulence, a reduced expression of several SIX (Secreted In Xylem) genes, the best studied family of effectors in F. oxysporum, and lower levels of SGE1 (Six Gene Expression 1) mRNA, the presumptive regulator of SIX expression. Moreover, the knockdown mutants showed a pattern of colonization of the host plant similar to that displayed by strains devoid of FTF1 copies (weakly virulent strains). Gene knockout of FTF2 also resulted in a reduction in virulence, but to a lesser extent. These results demonstrate the role of the FTF gene expansion, mostly the FTF1 paralogues, as a regulator of virulence in F. oxysporum and suggest that the control of effector expression is the mechanism involved. |
| Databáze: | OpenAIRE |
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