A nuclease-toxin and immunity system for kin discrimination in Myxococcus xanthus
| Autor: | Ze-shuo Li, Zheng Zhang, Yue-zhong Li, Ya Liu, Mian Nabeel Anwar, Wei Hu, Ya Gong, Xiu-wen Zhou |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Myxococcus xanthus 030106 microbiology Mutant Bacterial Toxins medicine.disease_cause Microbiology 03 medical and health sciences Bacterial Proteins Immunity medicine Escherichia coli Gene Ecology Evolution Behavior and Systematics Type VI secretion system Sequence Deletion Genetics Nuclease Deoxyribonucleases biology biology.organism_classification 030104 developmental biology biology.protein Bacteria |
| Zdroj: | Environmental microbiology. 20(7) |
| ISSN: | 1462-2920 |
| Popis: | The use of toxin to attack neighbours and immunity proteins to protect against toxin has been observed in bacterial conflicts, including kin discrimination. Here, we report a novel nuclease-toxin and its immunity protein function in the colony-merger incompatibility, a kind of bacterial kin discrimination, in Myxococcus xanthus DK1622. The MXAN_0049 gene was determined to be a genetic determinant for colony-merger incompatibility, and the incompatibility could be eliminated by deletion of the upstream co-transcribed MXAN_0050 gene. We demonstrated that the MXAN_0050 protein was a nuclease, and MXAN_0049 protein was able to bind to MXAN_0050 to block nuclease activity in vitro. Expression of MXAN_0050 in Escherichia coli inhibited cellular growth, and the inhibition effect could be recovered by co-expression of MXAN_0049. We found that deletion of the PAAR-encoding gene (MXAN_0044) or the type VI secretion system led to the colony-merger and co-existence with the ΔMXAN_0049 mutant, suggesting that they were associated with colony-merger incompatibility. Homologues of the nuclease-toxin and cognate immunity pair are widely distributed in bacteria. We propose a simplified model to explain the kin discrimination mechanism mediated by the nuclease-toxin and immunity protein.© 2018 Society for Applied Microbiology and John Wiley & Sons Ltd. |
| Databáze: | OpenAIRE |
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