Mage-3 and influenza-matrix peptide-specific cytotoxic T cells are inducible in terminal stage HLA-A2.1+ melanoma patients by mature monocyte-derived dendritic cells
| Autor: | Rod Dunbar, Beatrice Schuler-Thurner, Jürgen Knop, Ina Haendle, Claudia Röder, Eckhart Kämpgen, Armin Bender, Helmut Jonuleit, Gerhard Schuler, Christian Maczek, Vincenzo Cerundolo, Eva-B. Bröcker, P. von den Driesch, Petra Keikavoussi, Waltraud Leisgang, Alexander Enk, Detlef Dieckmann |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
CD4-Positive T-Lymphocytes
Cytotoxicity Immunologic medicine.medical_treatment Injections Subcutaneous Immunology Immunization Secondary Epitopes T-Lymphocyte CD8-Positive T-Lymphocytes Lymphocyte Activation Cancer Vaccines Monocytes Viral Matrix Proteins Antigens Neoplasm Tetanus Toxoid Immunology and Allergy Medicine Cytotoxic T cell Humans Melanoma Cells Cultured business.industry Toxoid Cell Differentiation Dendritic cell Dendritic Cells medicine.disease Neoplasm Proteins Immunization Injections Intravenous Intercellular Signaling Peptides and Proteins business Peptides Adjuvant CD8 Ex vivo T-Lymphocytes Cytotoxic |
| Zdroj: | Scopus-Elsevier |
| Popis: | Dendritic cell (DC) vaccination, albeit still in an early stage, is a promising strategy to induce immunity to cancer. We explored whether DC can expand Ag-specific CD8+ T cells even in far-advanced stage IV melanoma patients. We found that three to five biweekly vaccinations of mature, monocyte-derived DC (three vaccinations of 6 × 106 s.c. followed by two i.v. ones of 6 and 12 × 106, respectively) pulsed with Mage-3A2.1 tumor and influenza matrix A2.1-positive control peptides as well as the recall Ag tetanus toxoid (in three of eight patients) generated in all eight patients Ag-specific effector CD8+ T cells that were detectable in blood directly ex vivo. This is the first time that active, melanoma peptide-specific, IFN-γ-producing, effector CD8+ T cells have been reliably observed in patients vaccinated with melanoma Ags. Therefore, our DC vaccination strategy performs an adjuvant role and encourages further optimization of this new immunization approach. |
| Databáze: | OpenAIRE |
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