SSR marker-based genetic characterization of Turkish oriental tobaccos

Autor: Ibrahim Saygili, Nejdet Kandemir, Ahmet Kinay, Selim Aytac, Ali Kemal Ayan
Rok vydání: 2022
Předmět:
Zdroj: Molecular Biology Reports. 49:11351-11358
ISSN: 1573-4978
0301-4851
Popis: Background Turkey is one of the traditional oriental tobacco producing countries. Oriental tobaccos produced in Turkey are ecotypes specific to certain regions, local varieties and landraces, and they have unique characteristics. The present study was conducted to reveal the general and interregional genetic diversity levels of tobacco genotypes collected from different regions of Turkey based on DNA markers. Methods and Results 319 ecotypes/lines of Turkish Oriental Tobaccos collected from different regions (Aegean Region, Marmara Region, Karadeniz Region, and East and Southeast Anatolia Regions) of Turkey and cultivars Xanthi 81, Xanthi 2A, Canik Sitmasuyu, Katerini, Canik 190-5 and NC 55 were subjected to SSR marker analysis. A total of 89 alleles were obtained from 21 markers examined, and the average number of alleles per marker was 4.05. Nineteen polymorphic SSR markers find outed 314 unique genotypes among 319 tobacco plants evaluated. In the dendrogram constructed SSR marker data, genotypes were divided into six clades in account geographic regions and levels of genetic diversity. Conclusions The fact that only five of these genotypes were the same as another, in other words, the presence of 314 unique genotypes among 319 tobacco plants evaluated was another finding indicating the high level of genetic diversity in Turkish tobaccos. These genotypes could be tobaccos of different origins, and they merit further agronomic and technological characterizations.
Databáze: OpenAIRE
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