A src-like kinase activates outwardly rectifying chloride channels in CFTR-defective lymphocytes
| Autor: | Ulrich Wieland, Luzia Heil, Florian Lang, Tilmann Laun, Albecht Lepple-Wienhues, Martin Stern |
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| Rok vydání: | 2001 |
| Předmět: |
Cystic Fibrosis
Cell volume Src-Like Kinase Cystic Fibrosis Transmembrane Conductance Regulator Biochemistry Cystic fibrosis Chloride chemistry.chemical_compound Chloride Channels Cyclic AMP Genetics medicine Humans Cyclic adenosine monophosphate Lymphocytes Molecular Biology Chemistry Osmolar Concentration medicine.disease Cell biology Electrophysiology src-Family Kinases Lymphocyte Specific Protein Tyrosine Kinase p56(lck) Chloride channel Tyrosine kinase Biotechnology medicine.drug |
| Zdroj: | The FASEB Journal. 15:927-931 |
| ISSN: | 1530-6860 0892-6638 |
| Popis: | Defective activation of chloride channels is a hallmark of cystic fibrosis (CF). Recently we have described activation of a volume-sensitive, outwardly rectifying chloride conductance (I(OR)) through the src-like tyrosine kinase p56(lck). Here we show that p56(lck) activates I(OR) independently of CFTR. In lymphocytes from healthy donors, chloride channels could be opened by either intracellular cAMP, p56(lck) or osmotic swelling. In CF lymphocytes, p56(lck) and cell swelling but not cAMP could activate chloride channels. Regulation of I(OR) by p56(lck) thus represents an alternative pathway of stimulating membrane chloride conductance that is left intact in cystic fibrosis. |
| Databáze: | OpenAIRE |
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