Glucagon-stimulated but not isoproterenol-stimulated glucose formation inhibition by interleukin-6 in primary cultured rat hepatocytes
| Autor: | Bruno Christ, Quaas M, Stümpel F |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
STAT3 Transcription Factor medicine.medical_specialty Glycogenolysis Endocrinology Diabetes and Metabolism Clinical Biochemistry Glucagon binding Biology Biochemistry Glucagon 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Internal medicine medicine Cyclic AMP Receptors Glucagon Glucose homeostasis Animals Rats Wistar Cells Cultured 030304 developmental biology 0303 health sciences Glycogen Interleukin-6 Biochemistry (medical) Isoproterenol General Medicine Rats Glucose chemistry Gluconeogenesis Hepatocytes Phosphoenolpyruvate Carboxykinase (GTP) Phosphoenolpyruvate carboxykinase Glucagon receptor 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme. 37(11) |
| ISSN: | 0018-5043 |
| Popis: | During prolonged sepsis, impairment of glucose supply by the liver leads to hypoglycemia. Our aim was to investigate whether proinflammatory cytokine interleukin-6, a major mediator of the hepatic acute phase reaction, could contribute to this impairment by inhibiting hepatic glucose production stimulated by glucagon or isoproterenol in rat hepatocytes. Interleukin-6 inhibited the stimulation of glucose formation from glycogen by glucagon but not by isoproterenol in cultured rat hepatocytes. This was confirmed in the perfused rat liver. In cultured hepatocytes, the increase in cyclic adenosine-3',5'-monophosphate formation by glucagon was inhibited by interleukin-6, which was probably due to attenuation of glucagon binding to the glucagon receptor. The increase in cyclic adenosine-3',5'-monophosphate stimulated by isoproterenol was not affected by interleukin-6. However, the cytokine inhibited both expression of the key gluconeogenic control enzyme, phosphoenolpyruvate carboxykinase, stimulated by glucagon and isoproterenol. Thus, while increased glucose demand during the acute-phase reaction might initially be accomplished by catecholamine-mediated stimulation of glucose formation from glycogen, inhibition of gluconeogenesis by interleukin-6 may contribute to the impairment of glucose homeostasis during the prolonged acute phase reaction. |
| Databáze: | OpenAIRE |
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