Validation of saliva and urine use and sampling time on the doubly labelled water method to measure energy expenditure, body composition and water turnover in male and female cats
| Autor: | Aulus Cavalieri Carciofi, Eduardo Ferriolli, Gener Tadeu Pereira, Fernanda S. Mendonça, I.L.S. Senhorello, Francine M. Peres, Camila Goloni, Ludmilla G. Di Santo, Bruna Agy Loureiro, Karina Pfrimer |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Federal da Paraíba (UFPB), Universidade de São Paulo (USP) |
| Rok vydání: | 2020 |
| Předmět: |
Male
Saliva 040301 veterinary sciences Less invasive Medicine (miscellaneous) Urine Oxygen Isotopes 0403 veterinary science Animal science Body Water Felines Fatty mass Animals 2H Nutrition and Dietetics CATS Chemistry 0402 animal and dairy science 04 agricultural and veterinary sciences Energy metabolism Deuterium 040201 dairy & animal science Energy expenditure Lean body mass Body Composition Cats Composition (visual arts) Female Sampling time Energy Metabolism |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1475-2662 |
| Popis: | Less invasive protocols are necessary to study energy expenditure (EE) of cats living in homes for expressing their normal living conditions. The present study compared sampling times and the use of saliva, urine and blood to measure2H and18O to apply the doubly labelled water method. In the first study, four cats were used to evaluate the enrichment (2, 4, 6, 7 and 8 h) and elimination (2, 4, 6, 8, 10, 12, 14, 16, 18 and 20 d) of2H and18O (subcutaneously injected). The maximum enrichment was after 5 h (R20·82) of injection, with an Ln linear elimination of both isotopes (P< 0·001;R20·99). The results of EE were similar, regardless of the sampling time used (P= 0·999). In the second study, seven male cats and seven female cats were used. Before and after isotope injection (5 h, 7 d, 10 d and 14 d), blood, saliva and urine were collected. Isotope enrichment was lower in urine (P< 0·05) and at the similar level in blood and saliva. Isotope elimination was similar for all fluids (P< 0·473). The EE calculated with blood and saliva was similar but higher for urine (P= 0·015). According to Bland–Altman statistics, blood and saliva presented low bias and high correlation (P< 0·001), but this was not observed for urine (P= 0·096). Higher EE was observed for male cats (384 (se39) kJ/kg0·67per d) than for female cats (337 (se34) kJ/kg0·67per d;P< 0·05). The sampling time for the method is flexible, and saliva can be used as a substitute for blood. |
| Databáze: | OpenAIRE |
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