Fabrication and Characterization of Nanocomposite Hydrogel Based on Alginate/Nano-Hydroxyapatite Loaded with Linum usitatissimum Extract as a Bone Tissue Engineering Scaffold
| Autor: | Mahnaz Mohammadpour, Hadi Samadian, Nader Moradi, Zhila Izadi, Mahdieh Eftekhari, Masoud Hamidi, Amin Shavandi, Anthony Quéro, Emmanuel Petit, Cédric Delattre, Redouan Elboutachfaiti |
|---|---|
| Přispěvatelé: | Biologie des Plantes et Innovation - UR UPJV 3900 (BIOPI), Université de Picardie Jules Verne (UPJV)-Transfrontalière BioEcoAgro - UMR 1158 (BioEcoAgro), Université d'Artois (UA)-Université de Liège-Université de Picardie Jules Verne (UPJV)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Université d'Artois (UA)-Université de Liège-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Institut Pascal (IP), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA) |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Aquatic Organisms
alginate hydrogel Bone Regeneration Linum usitatissimum phenolics extract QH301-705.5 Alginates nano-hydroxyapatite Pharmaceutical Science Article Bone and Bones Cell Line Nanocomposites Flax Drug Discovery Humans [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering Biology (General) Pharmacology Toxicology and Pharmaceutics (miscellaneous) tissue engineering nanocomposite Tissue Scaffolds Plant Extracts technology industry and agriculture Durapatite |
| Zdroj: | Marine drugs Marine drugs, 2022, 20 (1), pp.20. ⟨10.3390/md20010020⟩ Marine Drugs; Volume 20; Issue 1; Pages: 20 Marine Drugs Marine Drugs, Vol 20, Iss 20, p 20 (2022) |
| ISSN: | 1660-3397 |
| DOI: | 10.3390/md20010020⟩ |
| Popis: | International audience; In the current paper, we fabricated, characterized, and applied nanocomposite hydrogel based on alginate (Alg) and nano-hydroxyapatite (nHA) loaded with phenolic purified extracts from the aerial part of Linum usitatissimum (LOH) as the bone tissue engineering scaffold. nHA was synthesized based on the wet chemical technique/precipitation reaction and incorporated into Alg hydrogel as the filler via physical cross-linking. The characterizations (SEM, DLS, and Zeta potential) revealed that the synthesized nHA possess a plate-like shape with nanometric dimensions. The fabricated nanocomposite has a porous architecture with interconnected pores. The average pore size was in the range of 100-200 mu m and the porosity range of 80-90%. The LOH release measurement showed that about 90% of the loaded drug was released within 12 h followed by a sustained release over 48 h. The in vitro assessments showed that the nanocomposite possesses significant antioxidant activity promoting bone regeneration. The hemolysis induction measurement showed that the nanocomposites were hemocompatible with negligible hemolysis induction. The cell viability/proliferation confirmed the biocompatibility of the nanocomposites, which induced proliferative effects in a dose-dependent manner. This study revealed the fabricated nanocomposites are bioactive and osteoactive applicable for bone tissue engineering applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|