Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction
| Autor: | Jia Jiang, Guoming Xie, Xiaoqiao Huangfu, Shikui Dong, Jinzhong Zhao, Li Yamin, Wang Liren, Tonghe Zhu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty Anterior cruciate ligament reconstruction Anterior cruciate ligament medicine.medical_treatment 0206 medical engineering Biomedical Engineering 02 engineering and technology Tendons Biomaterials Tissue engineering medicine Animals Transplantation Homologous Progenitor cell Decellularization Anterior Cruciate Ligament Reconstruction business.industry Hamstring Tendons Allografts musculoskeletal system 021001 nanoscience & nanotechnology medicine.disease 020601 biomedical engineering Tendon Cellular infiltration surgical procedures operative medicine.anatomical_structure Rabbits 0210 nano-technology business |
| Zdroj: | ACS Biomaterials Science & Engineering. 6:5700-5710 |
| ISSN: | 2373-9878 |
| DOI: | 10.1021/acsbiomaterials.0c00269 |
| Popis: | Anterior cruciate ligament (ACL) reconstruction with allografts is limited by high immunogenicity, poor cellularization, and delayed tendon-bone healing. Decellularized tendons (DAs) have been used as bioscaffolds to reconstruct ligaments with variable success. In the study, four kinds of decellularized allogeneic hamstring tendons were prepared and their microstructure and cytocompatibility were examined in vitro. The results showed that decellularized allografts neutralized by 5% calcium bicarbonate had typical reticular and porous microstructures with optical cytocompatibility. Tissue-engineering decellularized allografts (TEDAs) were prepared with the selected decellularized allografts and tendon stem/progenitor cells and used for ACL reconstruction in a rabbit model. Histological staining showed that the TEDAs promoted cellular infiltration and new vessel formation significantly and improved tendon-bone healing moderately compared to decellularized allografts. Better macroscopic scores and biomechanical results were observed in TEDA groups, but there were no significant differences between DA and TEDA groups at months 1, 2, and 3 postoperatively. Immunohistochemical data showed that the tissue-engineering decellularized allografts enhanced the expression of collagen I at each timepoint and collagen III at months 1 and 2. ELISA analysis showed that the tissue-engineering decellularized allografts reduced the secretion of IgE and IL-1β within 1 month and promoted the secretion of IL-2, IL-4, IL-10, and IL-17 after 1 month. The results showed that tissue-engineering decellularized allografts strengthened intra-articular graft remodeling significantly and provided moderate improvements in tendon-bone healing by creating more suitable immune responses than decellularized allografts. The study revealed that tissue-engineering decellularized allografts as a promising option for ACL reconstruction could achieve more favorable outcomes. |
| Databáze: | OpenAIRE |
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