Intra-cervical application of Misoprostol at estrus alters the content of cervical hyaluronan and the mRNA expression of follicle stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR) and cyclooxygenase-2 in the ewe
| Autor: | Sukanya Leethongdee, Rex J. Scaramuzzi, C.M. Kershaw-Young, Muhammad Khalid |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Uterus Gene Expression Cervix Uteri Biology Receptors Gonadotropin Estrus Food Animals Internal medicine medicine Animals RNA Messenger Hyaluronic Acid Small Animals Receptor Cervix Misoprostol In Situ Hybridization Feedback Physiological Estrous cycle Sheep Equine Artificial insemination luteinizing hormone/choriogonadotropin receptor Receptors LH medicine.anatomical_structure Endocrinology Cyclooxygenase 1 Receptors FSH Female Animal Science and Zoology Follicle-stimulating hormone receptor medicine.drug |
| Zdroj: | Theriogenology. 73:1257-1266 |
| ISSN: | 0093-691X |
| DOI: | 10.1016/j.theriogenology.2009.12.005 |
| Popis: | The complex anatomy the of ovine cervix limits the success of transcervical artificial insemination in sheep, but Misoprostol (a PGE(1) analogue) relaxes the cervix and facilitates transcervical artificial insemination. However, the mechanism by which Misoprostol causes cervical relaxation is not known. This study examined if intra-cervical Misoprostol altered the hyaluronan content and the mRNA expression of COX-2, LHR, or FSHR in the cervix of the estrus ewe. Estrus was synchronized in cyclic ewes with progestagen pessaries and 48 h after sponge removal ewes were treated intra-cervically with 0 (controls), 200, or 400 microg Misoprostol. Hyaluronan content was determined by ELISA and mRNA expression of LHR, FSHR, and COX-2 was analyzed by in situ hybridization using digoxigenin-11-uridine-5'-triphosphate labeled riboprobes. The hyaluronan content of the cervix was significantly higher in sheep that received 200 (P |
| Databáze: | OpenAIRE |
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