Molecular Methods for Identification of Monophasic Salmonella Typhimurium Strains
| Autor: | Monika Wasiak, Anna Chróst, Grzegorz Madajczak, Bozena Dera-Tomaszewska, Jolanta Szych |
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| Rok vydání: | 2015 |
| Předmět: |
DNA
Bacterial Salmonella typhimurium Microbiology (medical) Antigens Bacterial Salmonella Protein Array Analysis General Medicine Biology MOLECULAR BIOLOGY METHODS Serogroup medicine.disease_cause biology.organism_classification Applied Microbiology and Biotechnology Microbiology Bacterial genetics Bacterial protein Bacterial Proteins Salmonella enterica Multiplex polymerase chain reaction medicine Multiplex Polymerase Chain Reaction |
| Zdroj: | Polish Journal of Microbiology. 64:383-386 |
| ISSN: | 2544-4646 1733-1331 |
| Popis: | Two molecular biology methods were used to differentiate Salmonella enterica 1,4,[5],12:i:- strains: “Salmonella Check&Trace microarray” (CT) and multiplex PCR (mPCR). For 92 strains in CT result “Salmonella 1,4,[5],12:i:-“ were obtained. Those strains were confirmed in mPCR as monophasic fljB-lack Salmonella Typhimurium. For 17 strains, which in CT assay were recognized as Salmonella Typhimurium, the same identification was obtained in mPCR. Reference Salmonella strains: Lagos, Agama, Tsevie, Glocester and Tumodi in CT were recognized as Salmonella genovar, in mPCR – as Salmonella O:4, H:i other than Salmonella Typhimurium, the same like Salmonella Farsta, recognized incorrectly in CT as Salmonella Typhimurium. |
| Databáze: | OpenAIRE |
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