Impaired hepatic bacterial clearance is reversed by surgical relief of obstructive jaundice
| Autor: | Jay L. Grosfeld, Mark J. Rodefeld, Walter J. Folkening, Rong Yang, Schmuel Katz |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
medicine.medical_specialty Kupffer Cells Spleen Gastroenterology Sepsis Distal Common Bile Duct Phagocytosis Internal medicine Escherichia coli medicine Animals Humans Lung Cholestasis Common bile duct business.industry Infant Newborn Rats Inbred Strains General Medicine Mononuclear phagocyte system Jaundice medicine.disease Rats medicine.anatomical_structure Liver Biliary tract Choledochostomy Pediatrics Perinatology and Child Health Surgery medicine.symptom business Ligation |
| Zdroj: | Journal of Pediatric Surgery. 26:401-406 |
| ISSN: | 0022-3468 |
| DOI: | 10.1016/0022-3468(91)90986-4 |
| Popis: | Sepsis is a major cause of morbidity and mortality in infants with cholestatic jaundice. Previous studies have shown that biliary obstruction in rats causes a significant decrease in hepatic phagocytosis of viable Escherichia coli. This study tests this hypothesis and further evaluates whether the impaired function of the reticuloendothelial system of the liver (Kupffer cells) can be reversed by the relief of the biliary obstruction. Male Sprague-Dawley rats (weighing 140 to 150 g) were placed in three groups. Group I (n = 10) consisted of sham-operated controls. In Group II (n = 30), ligation and division of distal common bile duct (CDL) was performed. Group III (n = 30) underwent choledochoduodenostomy 2 weeks following ligation and division of common bile duct. At 1, 2, and 3 weeks following the operation, 10(9) 35S-radiolabeled viable E coli were injected intravenously via the tail vein. At 10 minutes, bacterial distribution in the liver, spleen, kidneys, and lungs was determined. Tissue samples (50 to 100 mg) from each organ were processed for liquid scintillation counting. The final distribution of bacteria was calculated from the input specific activity (dpm/bacteria) and expressed as the mean percentage of injected viable E coli per gram of tissue and per total organ weight. There was a significant decrease in the trapping of bacteria by the liver's Kupffer cells in rats in group II, at 2 and 3 weeks following CDL (45.0% +/- 14.0% and 15.1% +/- 4.9%, respectively, v controls 75.9% +/- 13.7%; P less than .005).(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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