Reduced constitutive 8-oxoguanine-DNA glycosylase expression and impaired induction following oxidative DNA damage in the tuberin deficient Eker rat
| Autor: | Serrine S. Lau, Terrence J. Monks, Minh N. Phan, Sonal K. Patel, Donghui Li, Samy L. Habib |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Male
congenital hereditary and neonatal diseases and abnormalities Cancer Research Tumor suppressor gene Mutant Fluorescent Antibody Technique Biology medicine.disease_cause Kidney Rats Mutant Strains hemic and lymphatic diseases Gene expression Tuberous Sclerosis Complex 2 Protein medicine Electrochemistry Animals neoplasms N-Glycosyl Hydrolases Chromatography High Pressure Liquid Mutation Tumor Suppressor Proteins Wild type General Medicine Molecular biology Glutathione nervous system diseases Hydroquinones Rats Repressor Proteins Oxidative Stress Mechanism of action DNA-Formamidopyrimidine Glycosylase DNA glycosylase embryonic structures medicine.symptom Oxidative stress DNA Damage |
| Zdroj: | Carcinogenesis. 24(3) |
| ISSN: | 0143-3334 |
| Popis: | The Tsc-2 tumor suppressor gene encodes the protein tuberin, a multi-functional protein with sequence homology to the GTPase activating protein (GAP) for Rap1. Mutations in the Tsc-2 gene are associated with the development of renal tumors. The Eker rat (Tsc-2(EK/+)) bears a mutation in one allele of the Tsc-2 gene, which predisposes these animals to renal cancer. Treatment of wild-type (Tsc-2(+/+)) and mutant (Tsc-2(EK/+)) Eker rats with 2,3,5-tris-(glutathion-S-yl)hydroquinone (TGHQ; 7.5 micro mol/kg. i.v.), a potent redox active and nephrotoxic metabolite of hydroquinone increases the incidence of renal tumors only in animals carrying the mutant Tsc-2(EK/+) allele. We now show that the constitutive expression of 8-oxoguanine-DNA glycosylase (OGG1) in Tsc-2(EK/+) rats is three-fold lower than in wild-type Tsc-2(+/+) rats. Moreover, treatment of wild-type and mutant Eker rats with TGHQ greatly increases 8-oxo-deoxyguanosine (8-oxo-dG) levels within the outer stripe of the outer medulla. Tsc-2(EK/+) rats, with lower constitutive renal OGG1 expression, experience substantially higher levels of 8-oxo-dG than do wild type Tsc-2(+/+) rats. Interestingly, whereas OGG1 expression was rapidly (4 h) induced in Tsc-2(+/+) rats following exposure to TGHQ, it was significantly reduced in Tsc-2(EK/+) rats. The combination of the higher constitutive expression of OGG1 in Tsc-2(+/+) rats, and its rapid induction in response to TGHQ treatment, coupled to the initial decrease in OGG1 expression in Tsc-2(EK/+) rats, results in Tsc-2(EK/+) OGG1 protein levels just 5% of those seen in Tsc-2(+/+) rats 8 h after treatment. Coincidentally, 8-oxo-dG levels in Tsc-2(+/+) rats 8 h after treatment with TGHQ are just 5% of those that occur in Tsc-2(EK/+) rats. The results indicate that the Tsc-2 gene influences constitutive OGG1 expression and the ability of OGG1 to respond to an oxidative stress, consistent with the proposal that Tsc-2 is an acute-phase response gene. In keeping with this view, acute TGHQ-induced cytotoxicity was greater in Tsc-2(EK/+) rats than in Tsc-2(+/+) rats. The mechanism(s) coupling tuberin expression to the regulation of OGG1 are not known and are under investigation. |
| Databáze: | OpenAIRE |
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