The Anti-mutated Citrullinated Vimentin Response Classifies Patients with Rheumatoid Arthritis into Broad and Narrow Responders
| Autor: | Martin Eggert, Robby Engelmann, Gunther Neeck, J. Brandt, Brigitte Mueller-Hilke, Andreas Krause, Kirsten Karberg |
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| Rok vydání: | 2009 |
| Předmět: |
musculoskeletal diseases
Immunology Arthritis medicine.disease_cause Autoantigens Peptides Cyclic Subclass Autoimmunity Arthritis Rheumatoid Rheumatology Antigen Antibody Specificity medicine Humans Vimentin Immunology and Allergy skin and connective tissue diseases Autoantibodies biology business.industry Autoantibody medicine.disease Immunoglobulin G Rheumatoid arthritis biology.protein Mutated citrullinated vimentin Antibody business |
| Zdroj: | The Journal of Rheumatology. 36:2670-2674 |
| ISSN: | 1499-2752 0315-162X |
| DOI: | 10.3899/jrheum.081263 |
| Popis: | Objective.Autoantibodies against citrullinated peptide antigens (ACPA) are routinely determined to diagnose rheumatoid arthritis (RA) and are predictive of a more severe course of the disease. We here set out to address an involvement of ACPA in the pathogenesis of RA and investigated the recognition pattern of antibodies against 2 citrullinated antigens in more detail.Methods.The sera of 77 patients fulfilling the American College of Rheumatology criteria for RA were analyzed for subclass titers of anti-mutated citrullinated vimentin (MCV) and anticyclic citrullinated peptide (CCP) antibodies by combining subclass specific detection antibodies with commercially available CCP and MCV ELISA plates. Cross-reactivities between anti-MCV and anti-CCP antibodies were detected using a sequential ELISA system.Results.IgG1, IgG3, and IgG4 titers among anti-MCV and anti-CCP antibodies correlated significantly. Cross-reactivity of MCV-specific antibodies against CCP could be detected in 8 of 16 patients’ sera; however, cross-binding of MCV-specific IgG4 was weaker compared to total IgG.Conclusion.The inherent capacity of IgG4 to exchange F(ab) arms provides insight into the anti-MCV antibody diversity and suggests a classification of ACPA positive patients into broad and narrow responders. |
| Databáze: | OpenAIRE |
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