Optical fiber LPG biosensor integrated microfluidic chip for ultrasensitive glucose detection
| Autor: | A. Ping Zhang, Shaorui Gao, Ming-Jie Yin, Xue-song Ye, Bo-bo Huang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Optical fiber
Materials science Nanotechnology 02 engineering and technology 01 natural sciences Article law.invention ocis:(050.2770) Gratings law Glucose oxidase Fiber ocis:(280.1415) Biological sensing and sensors Optical fiber cable Microchannel ocis:(060.2370) Fiber optics sensors biology 010401 analytical chemistry 021001 nanoscience & nanotechnology Chip Atomic and Molecular Physics and Optics 0104 chemical sciences Fiber optic sensor biology.protein 0210 nano-technology Biosensor ocis:(160.1435) Biomaterials Biotechnology |
| Zdroj: | Biomedical Optics Express |
| ISSN: | 2156-7085 |
| DOI: | 10.1364/boe.7.002067 |
| Popis: | An optical fiber sensor integrated microfluidic chip is presented for ultrasensitive detection of glucose. A long-period grating (LPG) inscribed in a small-diameter single-mode fiber (SDSMF) is employed as an optical refractive-index (RI) sensor. With the layer-by-layer (LbL) self-assembly technique, poly (ethylenimine) (PEI) and poly (acrylic acid) (PAA) multilayer film is deposited on the SDSMF-LPG sensor for both supporting and signal enhancement, and then a glucose oxidase (GOD) layer is immobilized on the outer layer for glucose sensing. A microfluidic chip for glucose detection is fabricated after embedding the SDSMF-LPG biosensor into the microchannel of the chip. Experimental results reveal that the SDSMF-LPG biosensor based on such a hybrid sensing film can ultrasensitively detect glucose concentration as low as 1 nM. After integration into the microfluidic chip, the detection range of the sensor is extended from 2 µM to 10 µM, and the response time is remarkablely shortened from 6 minutes to 70 seconds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|