Isolation of free-living dinitrogen-fixing bacteria and their activity in compost containing de-inking paper sludge
| Autor: | Gabriel Lévesque, François-Philippe Chalifour, Danielle Prévost, Chantal J. Beauchamp |
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| Rok vydání: | 2006 |
| Předmět: |
Paper
Environmental Engineering Nitrogen Bioengineering engineering.material complex mixtures Microbiology Soil Nitrogen Fixation Pseudomonas Waste Management and Disposal Azotobacter Bacteria Sewage biology Renewable Energy Sustainability and the Environment Compost Pseudomonas balearica fungi Temperature General Medicine Reference Standards biology.organism_classification engineering Nitrogen fixation Carbohydrate Metabolism Ink Sludge Pseudomonadaceae |
| Zdroj: | Bioresource Technology. 97:1002-1011 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2005.04.041 |
| Popis: | Knowledge of the microbiology of dinitrogen (N2)-fixing bacteria in compost rich in de-inking paper sludge (DPS) is limited. Dinitrogen (N2)-fixing bacteria from DPS composts were isolated and studied for their N2-fixing activity in vitro and in vivo. Two Gram-negative N2-fixing isolates were identified as Pseudomonas. At 20 degrees C, both isolates revealed that N2-fixing activity was higher than that of three arctic Pseudomonas strains. Their N2-fixing activity was found to occur between 18 and 25 degrees C, a pattern that was similar to the reference isolate Azotobacter ATCC 7486. Composts successfully showed N2-fixing activity after carbohydrate amendments both with and without inoculation of a N2-fixing isolate. These results suggest that DPS composts support N2-fixing bacteria and that N2-fixing activity is dependent on a usable carbohydrate source. |
| Databáze: | OpenAIRE |
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